Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

86 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Abaseed-ABA signaling in Arabidopsis seeds

ABSTRACT: ra14-04_nced - abaseed - Identification of abscisic acid (ABA) signaling factors and targets in Arabidopsis developing seeds by comparing ABA deficient and wild type genotypes. Transcriptome comparison of developing seeds harvested at 14 DAP from two ABA-deficient mutants (nced2 nced5 nced9 and nced2 nced5 nced6 nced9) and wild type (Columbia-0). 6 dye-swaps - gene knockout, genomic comparison.

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Soubigou-Taconnat Ludivine

PROVIDER: E-GEOD-68088 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

myb118 mutation; Identification of genes misregulated in developing seeds of Arabidopsis in response to the myb118 mutation.

Project description:ra12-06_myb118; myb118 mutation What are the genes over expressed or down regulated in seeds in response to a myb118 mutation? Plants of the different genotypes (wild-type, myb118 mutant, myb118 antisense lines named 0E3 and T16) were grown in a green house, their flower were tagged and seeds were harvested 10 days after anthesis. Seeds were extracted from the siliques, stored at -80Â°C prior to RNA extraction. 6 dye-swap - normal vs transgenic comparaison

2013-12-01 | E-GEOD-48180 | biostudies-arrayexpress

complex i-Analysis of complex I mutants

Project description:rs12-03_complex-i - complex i - Can we identify differences between mutants affecte din different subunits of complex I - direct comparison between col-0 (wild type) and 4 mutants, each mutants having different levels of residual complex I. Three biological replicates were processed 12 dye-swap - gene knock out The levels of residual complex I for the 4 mutants are as follows: mwfe (5%), 18K (traces), ca2 (10%), and 51K (0%).

2014-09-10 | E-GEOD-60533 | biostudies-arrayexpress

Comparison after 4 and 52 h (Chloro-Sap)

Project description:cea12-02_chlorosap - comparison after 4 and 52h - To observe the effects of guanosine penta/tetraphosphate on gene expression - Plants of four different genotypes (43A10.6, 44B1.3, H1 P3.2 and Col-0) were grown for ten days on MS/2 plates with 1% sucrose under 80ÂµE light in long-day conditions (16 hours of light, 8 hours of dark). Plants were treated at 10:30 in the morning (Time 0; 10:30 is 3 and a half hours after the onset of the light period) by flooding with 30uM dexamethasone (in aqueous solution with 0,3 % DMSO and 0,033 % Silwet L-77) for 5 minutes . Samples were taken at 4 hrs and 52 hrs after T0. Aerial parts of the seedlings were rapidly harvested and frozen in liquid nitrogen. 9 dye-swap - normal vs transgenic comparaison,time course

2014-11-01 | E-GEOD-62656 | biostudies-arrayexpress

bHLH39 overexpression

Project description:au13-06_fit - bhlh39 overexpression - Analyse the responses to bHLH39 overexpression. bHLH39 is one of the transcription factors regulating iron homeostasis responses. - Six day-old seedlings (wild type and bHLH39 overexpressor) were grown on Hoagland medium. Full seedlings were harvested for analysis. 21 dye-swap - normal vs transgenic comparaison

2015-02-14 | E-GEOD-65934 | biostudies-arrayexpress

plant rabt nÂ°1-Plant RABT nÂ°1

Project description:adt12-01_uprt - plant rabt nÂ°1 - Impact of RNA labeling by Plant RABT on plant transcriptome - Determine the incidence of RNA marking by the RABT method Plantation on the plants transcriptome. In several studies Clearly et al. have described a method referred as "4TU tagging" which can be use to study mRNA synthesis and decay either in a mixed population of cells or in a specific cell type (Cleary, Meiering et al. 2005, Zeiner, Cleary et al. 2008, Miller, Robinson et al. 2009, Rabani et al., 2011). Through the specific cell type expression in drosophila and mammalian cells of theToxoplasma gondii uracil PhosphoribosylTransferase activity and the metabolization of the uracil analog 4-thiouracil (4 TU), mRNA were selectively tagged, purified and used in microarray based analysis. 6 dye-swap - treated vs untreated comparison

2014-09-10 | E-GEOD-60532 | biostudies-arrayexpress

rnapaths--3_02/2012-RNAPATHS-3

Project description:blanc-08-01_2012_01_rnapaths_03 - rnapaths--3_02/2012 - Identify the transcript overlap and specificity between the PTGS and decapping/exoribonuclease pathways b identifying transcripts that are significantly changed in double mutants versus single mutants, and transcripts that are commonly changed among the single and double mutants compared to WT. - Identify transcripts that are significantly changed in double mutants (L1 vcs sgs2) (xrn4-5/sgs3-11) versus their respective single mutants (L1 vcs and L1 sgs2) (xrn4-5 and sgs3-11) , and identify transcripts that are changed among the single and double mutants compared to WT (Col) reference or to mutant L1 reference. 20 dye-swap - genotype comparaison

2012-07-12 | E-GEOD-38903 | biostudies-arrayexpress

cpsf30 mips1 transcriptome-Cell death control by CPSF30

Project description:au12-06_cpsf30 - cpsf30 mips1 transcriptome - How a mutation in the CPSF30 is able to suppress the cell death phenotype observed in the mips1-1 Arabidopsis mutant? - All the plants were cultivated in the same hanging conditions 10days in "short Days" then in long Days. The takings were later made 4days in "long Days" what corresponds to the appearance of the symptoms of cellular death at the mutant mips1-1. On the other hand, the gene CPSF30 codes for two transcribed. We have a double mutant mips1 cpsf30 complemented with the transcribed young. 6 dye-swap - genotype comparaison

2014-02-01 | E-GEOD-53247 | biostudies-arrayexpress

4 mutants-Transcriptome sup62 and cpsf30 mpk4

Project description:au13-05_sup62 - 4 mutants - Transcriptome analysis of the mutant sup62, cpsf30, two suppressors of programmed cell death in A. thaliana - Plants Col-0, mips1, sup62 and sup62 mips1 were grown for 3 weeks in Short-Day condition and subsequently transfered in Long-Day condition. Rosette leaves were sampled after 4 days in Long-Day condition. 8 dye-swap - gene knock out,genotype comparaison

2015-04-01 | E-GEOD-67010 | biostudies-arrayexpress

transcript modification by myb1 interactants-MYB1’s partners

Project description:au13-14_myb1 - transcript modification by myb1 interactants - Transcription modification produced by transgenes - We produced 5 transgenic lines, overexpressing Eg MYB1,Eg MYB1+ EgOVATE, EgMYB1+EgHISTONE, EgHISTONE, EgOVATE and the CONTROL with the empty vectors. We have 2 lines of each construction. Plants were grown in short day conditions and we sampled the inflorescence stem. 10 dye-swap - normal vs transgenic comparaison

2015-05-01 | E-GEOD-68024 | biostudies-arrayexpress

cdt1_bis; Transcriptome comparison of the crl mutant and CDT1-RNAi line

Project description:au13-04_cdtbis; cdt1_bis crl mutants and CDT1-RNAi lines have very similar macroscopic phenotypes as well as identical defects in plastid division and biogenesis. Our goal wwas to determine how much of these similarities originated from similar alterations of gene expression. Plantlets of crl mutant and CDT1-RNAi lines were grown in vitro on MS1/2 medium for 14 days. CDT1-RNAi lines were compared to the corresponding wild-type (Ws), whereas crl mutants were compared to their wild-type siblings and are in the Col0 ecotype. 4 dye-swap - normal vs rnai mutant comparaison, normal vs transgenic comparaison

2013-09-01 | E-GEOD-48465 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data