ABSTRACT: ra14-04_nced - abaseed - Identification of abscisic acid (ABA) signaling factors and targets in Arabidopsis developing seeds by comparing ABA deficient and wild type genotypes. Transcriptome comparison of developing seeds harvested at 14 DAP from two ABA-deficient mutants (nced2 nced5 nced9 and nced2 nced5 nced6 nced9) and wild type (Columbia-0). 6 dye-swaps - gene knockout, genomic comparison.
Project description:ra12-06_myb118; myb118 mutation What are the genes over expressed or down regulated in seeds in response to a myb118 mutation? Plants of the different genotypes (wild-type, myb118 mutant, myb118 antisense lines named 0E3 and T16) were grown in a green house, their flower were tagged and seeds were harvested 10 days after anthesis. Seeds were extracted from the siliques, stored at -80°C prior to RNA extraction. 6 dye-swap - normal vs transgenic comparaison
Project description:rs12-03_complex-i - complex i - Can we identify differences between mutants affecte din different subunits of complex I - direct comparison between col-0 (wild type) and 4 mutants, each mutants having different levels of residual complex I. Three biological replicates were processed 12 dye-swap - gene knock out The levels of residual complex I for the 4 mutants are as follows: mwfe (5%), 18K (traces), ca2 (10%), and 51K (0%).
Project description:cea12-02_chlorosap - comparison after 4 and 52h - To observe the effects of guanosine penta/tetraphosphate on gene expression - Plants of four different genotypes (43A10.6, 44B1.3, H1 P3.2 and Col-0) were grown for ten days on MS/2 plates with 1% sucrose under 80µE light in long-day conditions (16 hours of light, 8 hours of dark). Plants were treated at 10:30 in the morning (Time 0; 10:30 is 3 and a half hours after the onset of the light period) by flooding with 30uM dexamethasone (in aqueous solution with 0,3 % DMSO and 0,033 % Silwet L-77) for 5 minutes . Samples were taken at 4 hrs and 52 hrs after T0. Aerial parts of the seedlings were rapidly harvested and frozen in liquid nitrogen. 9 dye-swap - normal vs transgenic comparaison,time course
Project description:au13-06_fit - bhlh39 overexpression - Analyse the responses to bHLH39 overexpression. bHLH39 is one of the transcription factors regulating iron homeostasis responses. - Six day-old seedlings (wild type and bHLH39 overexpressor) were grown on Hoagland medium. Full seedlings were harvested for analysis. 21 dye-swap - normal vs transgenic comparaison
Project description:adt12-01_uprt - plant rabt n°1 - Impact of RNA labeling by Plant RABT on plant transcriptome - Determine the incidence of RNA marking by the RABT method Plantation on the plants transcriptome. In several studies Clearly et al. have described a method referred as "4TU tagging" which can be use to study mRNA synthesis and decay either in a mixed population of cells or in a specific cell type (Cleary, Meiering et al. 2005, Zeiner, Cleary et al. 2008, Miller, Robinson et al. 2009, Rabani et al., 2011). Through the specific cell type expression in drosophila and mammalian cells of theToxoplasma gondii uracil PhosphoribosylTransferase activity and the metabolization of the uracil analog 4-thiouracil (4 TU), mRNA were selectively tagged, purified and used in microarray based analysis. 6 dye-swap - treated vs untreated comparison
Project description:blanc-08-01_2012_01_rnapaths_03 - rnapaths--3_02/2012 - Identify the transcript overlap and specificity between the PTGS and decapping/exoribonuclease pathways b identifying transcripts that are significantly changed in double mutants versus single mutants, and transcripts that are commonly changed among the single and double mutants compared to WT. - Identify transcripts that are significantly changed in double mutants (L1 vcs sgs2) (xrn4-5/sgs3-11) versus their respective single mutants (L1 vcs and L1 sgs2) (xrn4-5 and sgs3-11) , and identify transcripts that are changed among the single and double mutants compared to WT (Col) reference or to mutant L1 reference. 20 dye-swap - genotype comparaison
Project description:au12-06_cpsf30 - cpsf30 mips1 transcriptome - How a mutation in the CPSF30 is able to suppress the cell death phenotype observed in the mips1-1 Arabidopsis mutant? - All the plants were cultivated in the same hanging conditions 10days in "short Days" then in long Days. The takings were later made 4days in "long Days" what corresponds to the appearance of the symptoms of cellular death at the mutant mips1-1. On the other hand, the gene CPSF30 codes for two transcribed. We have a double mutant mips1 cpsf30 complemented with the transcribed young. 6 dye-swap - genotype comparaison
Project description:au13-05_sup62 - 4 mutants - Transcriptome analysis of the mutant sup62, cpsf30, two suppressors of programmed cell death in A. thaliana - Plants Col-0, mips1, sup62 and sup62 mips1 were grown for 3 weeks in Short-Day condition and subsequently transfered in Long-Day condition. Rosette leaves were sampled after 4 days in Long-Day condition. 8 dye-swap - gene knock out,genotype comparaison
Project description:au13-14_myb1 - transcript modification by myb1 interactants - Transcription modification produced by transgenes - We produced 5 transgenic lines, overexpressing Eg MYB1,Eg MYB1+ EgOVATE, EgMYB1+EgHISTONE, EgHISTONE, EgOVATE and the CONTROL with the empty vectors. We have 2 lines of each construction. Plants were grown in short day conditions and we sampled the inflorescence stem. 10 dye-swap - normal vs transgenic comparaison
Project description:au13-04_cdtbis; cdt1_bis crl mutants and CDT1-RNAi lines have very similar macroscopic phenotypes as well as identical defects in plastid division and biogenesis. Our goal wwas to determine how much of these similarities originated from similar alterations of gene expression. Plantlets of crl mutant and CDT1-RNAi lines were grown in vitro on MS1/2 medium for 14 days. CDT1-RNAi lines were compared to the corresponding wild-type (Ws), whereas crl mutants were compared to their wild-type siblings and are in the Col0 ecotype. 4 dye-swap - normal vs rnai mutant comparaison, normal vs transgenic comparaison