Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Gene expression profiles of CD4+CD25+ Tregs from NOD and B6.H2g7 mice

ABSTRACT: The NOD (nonobese diabetic) mouse strain develops a characteristic autoimmune syndrome that closely resembles human type I diabetes. It has been suggested that NOD mice exhibit both numerical deficiency in CD4+CD25+ regulatory T cells (Treg) and reduced suppressive activity. We compared sorted CD4+CD25+ Tregs from the spleens of 6-7 week-old female NOD and nondiabetic B6.H2g7 mice. Tregs were 93±2% and 95±1% Foxp3+ in NOD and B6.H2g7 cells, respectively, on post-sort reanalysis. "Conventional" CD4+CD25- T cells (Tconv) are included as reference populations. Surprisingly, Treg "signature" is similar between the two strains, with only a few probesets that subtly deviate. Keywords: Cell population comparison from two mouse strains. For each strain (NOD and B6.g7), we analyzed two populations: CD4+CD25+ Treg and CD4+CD25- Tconv cells, for a total of four distinct populations. RNA from three mice were pooled for each replicate; there are three independent replicates for each population. After RMA normalization, intensity values were averaged across the three replicates and analyzed. We calculated the ratio of Treg/Tconv intensity values for each strain and compared the results.

ORGANISM(S): Mus musculus

SUBMITTER: CBDM Lab 

PROVIDER: E-GEOD-6813 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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The defect in T-cell regulation in NOD mice is an effect on the T-cell effectors.

D'Alise Anna Morena AM   Auyeung Vincent V   Feuerer Markus M   Nishio Junko J   Fontenot Jason J   Benoist Christophe C   Mathis Diane D  

Proceedings of the National Academy of Sciences of the United States of America 20081210 50

FoxP3(+) regulatory T cells (Tregs) protect against autoimmunity, type 1 diabetes (T1D) in particular, prompting the hypothesis that a deficiency in Tregs is a critical determinant of diabetes susceptibility in NOD mice. However, tests of this hypothesis have yielded contradictory results. We confirmed that NOD mice, compared with reference strains, do not have a primary deficit in Treg numbers in the lymphoid organs, whether in prediabetic mice of any age or in animals with recent-onset diabete  ...[more]

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