Real-time quantitative PCR analysis of cucumber mitogen activated protein kinase cascade genes under various stress conditions and plant hormone treatments

ABSTRACT: We used Bio-Rad laboratories, lnc. PCR assay panel to analyze expression levels of MAPK genes under various stress conditions and plant hormone treatments. Cucumber plants of the ‘Jinyan No.4’ cultivar were reared in growth chambers at 28 ± 1 °C with a photoperiod of 16 h light/8 h dark and light intensity of 400 μmol m−2 s−1. Three-week-old seedlings were used for all abiotic and biotic treatments. For heat or cold treatment, the seedlings were subjected to 35 ± 1 °C or 4 ± 1 °C conditions, respectively. The samples for RNA extraction were collected at 0, 1, 2, 4, and 8 h after treatment. For dehydration treatment, the leaves of plants were sampled at 0, 2, 4, and 6 d after watering stopped. For disease treatment, Pseudoperonospora cubensis (P.cubensis) was used to infect the seedlings,

ORGANISM(S): Cucumis sativus

SUBMITTER: Jie Wang 

PROVIDER: E-GEOD-68436 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress