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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Analyses of Early- and Late-scnRNAs of Tetrahymena

ABSTRACT: In this study, we demonstrated that there is a novel, unanticipated mechanism regulating programmed DNA elimination: a genome-wide trans-recognition network for IES identification. In this mechanism, Early-scnRNAs produced from Type-A IESs in the MIC identify not only the IESs from which they are derived but also other IESs in trans to trigger the cis-spreading of Late-scnRNA production in the IESs. This cis-spreading of Late-scnRNA production requires heterochromatin formation . Furthermore, these Late-scnRNAs can recognize other IESs in trans. This “chain reaction” of Late-scnRNA production by the trans-recognition network most likely provides strong robustness in DNA elimination by buffering cell-to-cell variability in the initial Early-scnRNA populations. 26 to 32-nt small RNAs from various mutants or from immuno precipitated with Argonaute proteins were analyzed by high-throughput sequencing

ORGANISM(S): Tetrahymena thermophila

SUBMITTER: Kazufumi Mochizuki

PROVIDER: E-GEOD-68455 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Analyses of Early- and Late-scnRNAs of Tetrahymena

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Tetrahymena thermophila

Project description:Analyses of Early- and Late-scnRNAs of Tetrahymena

| PRJNA282767 | ENA