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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Prim_Fibs

ABSTRACT: To determine changes in gene expression in primary fibroblasts undergoing replicative senescence, a direct comparison between early passage proliferating cells and senescent cells was performed. Frozen stocks of the primary fibroblasts (HMF3) from which the lines HMF3A and HMF3Dwt were derived (O'Hare, PNAS:98, 2001) were serially passaged twice through to senescence to yield 2 independent sets of RNA. A third set of RNA was from a different female donor (F1068). The three different sets of RNA were put on 4 arrays each. Data is also provided for F1068 fibroblasts at late passage. Keywords = fibroblast Keywords = senescence Keywords = breast

ORGANISM(S): Homo sapiens

SUBMITTER: Parmjit Jat

PROVIDER: E-GEOD-687 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Transcriptional networks and cellular senescence in human mammary fibroblasts.

Hardy K K Mansfield L L Mackay A A Benvenuti S S Ismail S S Arora P P O'Hare M J MJ Jat P S PS

Molecular biology of the cell 20041201 2

Senescence, the molecular program that limits the finite proliferative potential of a cell, acts as an important barrier to protect the body from cancer. Techniques for measuring transcriptome changes and for modulating their expression suggest that it may be possible to dissect the transcriptional networks underlying complex cellular processes. HMF3A cells are conditionally immortalized human mammary fibroblasts that can be induced to undergo coordinated senescence. Here, we used these cells in ...[more]

PMID: 15574883

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