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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Gene expression signatures that predict radiation exposure

ABSTRACT: This SuperSeries is composed of the following subset Series: GSE6871: Gene expression signatures that predict radiation exposure (human) GSE6873: Gene expression signatures that predict radiation exposure (mouse) Keywords: SuperSeries Refer to individual Series

ORGANISM(S): Mus musculus

SUBMITTER: Holly Dressman

PROVIDER: E-GEOD-6874 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Gene expression signatures that predict radiation exposure in mice and humans.

Dressman Holly K HK Muramoto Garrett G GG Chao Nelson J NJ Meadows Sarah S Marshall Dawn D Ginsburg Geoffrey S GS Nevins Joseph R JR Chute John P JP

PLoS medicine 20070401 4

<h4>Background</h4>The capacity to assess environmental inputs to biological phenotypes is limited by methods that can accurately and quantitatively measure these contributions. One such example can be seen in the context of exposure to ionizing radiation.<h4>Methods and findings</h4>We have made use of gene expression analysis of peripheral blood (PB) mononuclear cells to develop expression profiles that accurately reflect prior radiation exposure. We demonstrate that expression profiles can be ...[more]

PMID: 17407386

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Gene expression signatures that predict radiation exposure (mouse)

Project description:Gene expression analysis of peripheral blood leukocytes (PB MNCs) to develop expression profiles that accurately reflect prior radiation exposure. Keywords: Comparative, exposure dosage, C57BI6 Murine Irradiation Studies We have made use of gene expression analysis of peripheral blood mononuclear cells (PB MNCs) to develop expression profiles that accurately reflect prior radiation exposure. Importantly, we demonstrate that expression profiles can be developed that not only predict radiation exposure in mice but also distinguish the level of radiation exposure, ranging from 50 cGy to 1000 cGy. Likewise, a molecular signature of radiation response developed solely from irradiated human patient samples can predict and distinguish irradiated human PB samples from non-irradiated samples with an accuracy of 90%, sensitivity of 85% and specificity of 94%. We further demonstrate that a radiation profile developed in the mouse can correctly distinguish PB samples from irradiated and non-irradiated human patients with an accuracy of 77%, sensitivity of 82% and specificity of 75%. Taken together, these data demonstrate that molecular profiles can be generated which are highly predictive of different levels of radiation exposure in mice and humans. Mouse Dataset only

2010-06-11 | E-GEOD-6873 | biostudies-arrayexpress

Changes in Gene expression mediated by Cyclic Mechanical Stress in Trabecular Meshwork Cells

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Project description:Nrf2 (NF-E2-related factor-2) transcription factor regulates oxidative/xenobiotic stress response and also represses inflammation. However, the mechanisms how Nrf2 alleviates inflammation are still unclear. Here, we demonstrate that Nrf2 interferes with lipopolysaccharide-induced transcriptional upregulation of proinflammatory cytokines, including IL-6 and IL-1Î². ChIP-seq and ChIP-qPCR analyses revealed that Nrf2 binds to the proximity of these genes in macrophages and inhibits RNA Pol II recruitment. Further, we found that Nrf2-mediated inhibition is independent of the Nrf2 binding motif and reactive oxygen species level. Murine inflammatory models further demonstrated that Nrf2 interferes with IL6 induction and inflammatory phenotypes in vivo. Thus, contrary to the widely accepted view that Nrf2 suppresses inflammation through redox control, we demonstrate here that Nrf2 opposes transcriptional upregulation of proinflammatory cytokine genes. This study identifies Nrf2 as the upstream regulator of cytokine production and establishes a molecular basis for an Nrf2-mediated anti-inflammation approach. Gene expression in BMDMs obtained from wild-type and Keap1-CKO mice. In Keap1-CKO (Keap1 flox/flox::LysM-Cre) BMDMs, Nrf2 transcription factor is activated due to Keap1-deficiency. BMDMs were obtained by a culture of bone marrow cells in the presence of M-CSF for7 days. M1-activated BMDMs were obtained by stimulation with LPS and IFNg for 6 hours, while M2-activated BMDMs were obtained by a stimulation with IL-4 for 6 hours. Two independent BMDM cultures were performed, and each experiment contains samples obtained from one wild-type and one Keap1-CKO mice, respectively.

2016-05-12 | E-GEOD-71263 | biostudies-arrayexpress

Transcription profiling time series of Candida albicans treated with different doses of acetic acid

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2008-07-31 | E-MEXP-1645 | biostudies-arrayexpress

Transcription profiling of Candida albicans wild type and mnl1 cells after treatment with acetic acid

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2008-07-31 | E-MEXP-1641 | biostudies-arrayexpress

Transcription profiling of Candida dubliniensis and Candida albicans under stress conditions

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2008-12-01 | E-MEXP-1650 | biostudies-arrayexpress

Transcription profiling of Candida albicans wild type strain BWP17 and mutant hac1 treated with DTT or tunicamycin to investigate the effect of the Unfolded Protein Response of genome-wide expression patterns

Project description:Candida albicans wild type strain BWP17 and mutant hac1 were submitted either to DTT or tunicamycin, both agents being able to trigger the Unfolded Protein Response.

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Project description:effect of heatstress on wildtype and hsf1 mutant cells

2009-12-17 | E-MEXP-2044 | biostudies-arrayexpress

Transcription profiling wild type C. albicans after treatment with stress-inducing doses of acetic acid

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2008-07-31 | E-MEXP-1633 | biostudies-arrayexpress

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