Transcription profiling of human peripheral blood lymphocytes: comparison between melanoma patients and healthy controls

ABSTRACT: We focused on the major peripheral blood lymphocyte populations that may be involved in anti-tumor responses and negatively impacted by cancer, specifically CD8 T cells, CD4 T cells, B cells and CD56dim natural killer cells. The pure cell subsets were stringently sorted by flow cytometry from PBMC samples. Gene expression profiles of these cell populations from melanoma patients were compared to healthy controls. Experiment Overall Design: The raw data set contained 48 arrays: 6 healthy and 6 melanoma arrays for each of the 4 cell types. Two of the arrays had quality issues due to background noise and were excluded, leaving us with 46 arrays. Experiment Overall Design: On each array we used Cy3 to label a pool of two RNA samples from a pair of age and gender matched stage IV (American Join

ORGANISM(S): Homo sapiens

SUBMITTER: Peter Lee 

PROVIDER: E-GEOD-6887 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress