Human aldosterone producing cell clusters, zona glomerulosa, zona fasciculata, and zona reticularis, from adrenal glands

ABSTRACT: In order to analyze the transcriptome characteristics of aldosterone producing cell clusters (APCC) we compared transcript abundances of APCC, zona glomerulosa (ZG), zona fasciculata (ZF), and zona reticularis (ZR), from adrenal glands obtained from 4 kidney transplantation donors. The frozen adrenal glands in O.C.T. compound were cut into 7um sections, and every 10-th section immunostained for aldosterone synthase (CYP11B2). The remaining sections were stained with cresyl violet and used for laser-capture microdissection of tissue to use in the array assays. APCC and ZG samples were captured from CYP11B2 positive regions based on the CYP11B2-stained sections. ZF and ZR were captured from lipid-rich cells in the middle layer and compact cells outside of the medulla, respectively. RNA

ORGANISM(S): Homo sapiens

SUBMITTER: Rork Kuick 

PROVIDER: E-GEOD-68889 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress