Dazl is required for TET-mediated reprogramming to a naïve pluripotent state (I)

ABSTRACT: Embryonic stem cell (ESC) cultures display a heterogeneous gene expression profile, ranging from a pristine naïve pluripotent state to a primed epiblast state. While it is known that the addition of inhibitors of GSK3? and MEK (so-called 2i conditions) push ESC cultures towards a more homogeneous naïve pluripotent state, the molecular underpinnings of this naïve transition are not completely understood. Here we demonstrate that Dazl, a RNA-binding protein previously thought to be expressed specifically in developing primordial germ cells (PGCs), marks a subpopulation of ESCs in vitro that is actively transitioning toward naïve pluripotency. In the absence of Dazl expression, ESCs fail to induce proper expression of Tet enzymes required for 5-hydroxymethylation in 2i-culture conditions. As

ORGANISM(S): Mus musculus

SUBMITTER: Niels Geijsen 

PROVIDER: E-GEOD-69055 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress