Genome-wide profiling of nucleosome sensitivity and chromatin accessibility in D. melanogaster [MNase]

ABSTRACT: Nucleosome structure and positioning play pivotal roles in gene regulation, DNA repair and other essential processes in eukaryotic cells. Nucleosomal DNA is thought to be uniformly inaccessible to DNA binding and processing factors, such as MNase. Here, we show, however, that nucleosome accessibility and sensitivity to MNase varies. Digestion of Drosophila chromatin with two distinct concentrations of MNase revealed two types of nucleosomes: sensitive and resistant. MNase-resistant nucleosome arrays are less accessible to low concentrations of MNase, whereas MNase-sensitive arrays are degraded by high concentrations. MNase-resistant nucleosomes assemble on sequences depleted of A/T and enriched in G/C containing dinucleotides. In contrast, MNase-sensitive nucleosomes form on A/T rich seque

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Razvan Chereji 

PROVIDER: E-GEOD-69177 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress