Genome-wide maps of H3K36me3 in clear cell renal cell carcinoma (ccRCC) [ChIP-seq]

ABSTRACT: To address the need to study frozen clinical specimens using next-generation RNA, DNA, chromatin immunoprecipitation (ChIP) sequencing and protein analyses, we developed a biobank work flow to prospectively collect biospecimens from patients with renal cell carcinoma (RCC). We describe our standard operating procedures and work flow to annotate pathologic results and clinical outcomes. We report quality control outcomes, nucleic acid yields of our RCC submissions (N=16) to The Cancer Genome Atlas (TCGA) project, as well as newer discovery platforms by describing mass spectrometry analysis of albumin oxidation in plasma and 6 ChIP sequencing libraries generated from nephrectomy specimens after histone H3 lysine 36 trimethylation (H3K36me3) immunoprecipitation. From June 1, 2010, through Jan

ORGANISM(S): Homo sapiens

SUBMITTER: Jeff Nie 

PROVIDER: E-GEOD-69196 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress