Browse
Submit Data
Databases
API
Help

Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

5 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcription profiling of mouse FLT3+/CD11b+ Dendritic cell (DC) progenitor

ABSTRACT: FLT3+/CD11b+ Dendritic cell (DC) progenitor was amplified in vitro from mouse bone marrow.

ORGANISM(S): Mus musculus

SUBMITTER: Thomas Hieronymus

PROVIDER: E-GEOD-692 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Publications

Progressive and controlled development of mouse dendritic cells from Flt3+CD11b+ progenitors in vitro.

Hieronymus Thomas T Gust Tatjana C TC Kirsch Ralf D RD Jorgas Thorsten T Blendinger Gitta G Goncharenko Mykola M Supplitt Kamilla K Rose-John Stefan S Müller Albrecht M AM Zenke Martin M

Journal of immunology (Baltimore, Md. : 1950) 20050301 5

Dendritic cells (DC) represent key regulators of the immune system, yet their development from hemopoietic precursors is poorly defined. In this study, we describe an in vitro system for amplification of a Flt3(+)CD11b(+) progenitor from mouse bone marrow with specific cytokines. Such progenitor cells develop into both CD11b(+) and CD11b(-) DC, and CD8alpha(+) and CD8alpha(-) DC in vivo. Furthermore, with GM-CSF, these progenitors synchronously differentiated into fully functional DC in vitro. T ...[more]

PMID: 15728461

Similar Datasets

FLT3+/CD11b+ Dendritic cell (DC) progenitor

Project description:FLT3+/CD11b+ Dendritic cell (DC) progenitor was amplified in vitro from mouse bone marrow. Keywords: repeat sample

2004-09-30 | GSE692 | GEO

Mouse lung CD103+ and CD11b-high dendritic cell (DC) subsets

2010-03-15 | GSE17322 | GEO

DC differentiation from DC progenitor in vitro

Project description:FLT3+CD11b+ DC progenitor was amplified in vitro from mouse bone marrow. DC were differentiated using GM-CSF and analyzed at day 7 and day 10 of differentiation and after 16h TNFalpha stimulation at day 10 Keywords: time-course

2004-09-30 | GSE575 | GEO

Mouse lung CD103+ and CD11b-high dendritic cell (DC) subsets

Project description:Mouse lung CD11c+ dendritic cells are composed of 2 major DC subsets, the CD103+CD11b-low/intermediate DC (CD103+ DC) and the CD11b-highCD103- DC (CD11b-high DC). These 2 subsets are functionally distinct. Comparison of their functions showed CD103+ DC Microarray analysis was performed to compare the gene expression profiles of the 2 lung DC subsets in naÃ¯ve mice. Perfused lungs from 6-8-week naÃ¯ve BALB/cByJ mice were pooled and digested with collagenase D. CD11c+ cells were selected by anti-CD11c magnetic microbeads (Miltenyi) and stained by fluorochrome-conjugated mAb against I-A, CD103, CD11b, and CD11c plus 7-

2010-03-24 | E-GEOD-17322 | biostudies-arrayexpress

Mus musculus

Project description:FLT3+/CD11b+ Dendritic cell (DC) progenitor

| PRJNA87647 | ENA

Transcription profiling of mouse dendritic cell differentiation from dendritic cell progenitor in vitro

2008-06-14 | E-GEOD-575 | biostudies-arrayexpress

FLT3-ITD mutation expands dendritic cells and alters CD4+ T cells in acute myeloid leukemia

Project description:Fms-like tyrosine kinase 3 (FLT3) is a critical receptor for functional dendritic cell (DC) development. Mutations associated with FLT3 are commonly observed in acute myeloid leukemia (AML) patients. Internal tandem duplication (FLT3-ITD) results in ligand-independent constitutive signaling and promotes tumor survival. Basic characterization of dendritic cells in the context of AML is lacking, therefore we investigated how FLT3-ITD impacts DC homeostasis and what downstream affects on the immune system may be. During AML we found that patient bone marrow DC homesostasis is disrupted and in a mouse model of AML we found that T-bet- cDC2s are increased in vivo and both Treg and Th17 cells are increased. In vitro co-cultures of AML DCs and naive OT-II cells results in more Th17 phenotype by IL-17A secretion. We propose that in FLT3-ITD+ AML DC homeostasis is dysregulated and Th17 cells are increased as a result.

2023-08-23 | GSE238156 | GEO

Runx3 function in colon resident macrophages (RM) and CD11b+ dendritic cells (DC).

Project description:Runx3 function in colon resident macrophages (RM) and CD11b+ dendritic cells (DC).

| PRJNA561153 | ENA

Transcriptome profile of mouse lung dendritic cell subsets and macrophages.

Project description:Dendritic cell (DC) are critical initiators and regulators of immunity to pathogens, vaccines, tumors and tolerance to self. Mouse and human dendritic cells (DCs) are comprised of functionally specialized subsets, but precise interspecies correlation is currently incomplete and hampers the full translation of murine findings to human DC-based clinical therapies. In this study, we show that murine lung and gut lamina propria CD11b+ DC populations are comprised of two subsets: FLT3- and IRF4-dependent CD24+CD64- DCs and contaminating CSF-1R-dependent CD24-CD64+ macrophages. CD11b+CD24+CD64- DCs are instrumental in inducing Th17 cell immune response in the steady state and upon Aspergillus fumigatus challenge. We also identified human CD1c+CD11b+ DCs as the functional homologue of mouse mucosal CD11b+ DCs. Our findings highlight the conservation of key immune functions across species and aid the translation of murine studies to human DC immunobiology. The data for the associated human studies have been stored within GSE35459. Gene Expression from total RNA from specific mouse dendritic cell subsets purified by FACS

2013-05-29 | E-GEOD-46680 | biostudies-arrayexpress

Transcriptome profile of mouse lung dendritic cell subsets and macrophages.

2013-05-29 | GSE46680 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data