Transcriptome Profiling of Developing Murine Lens through RNA Sequencing

ABSTRACT: Purpose: Transcriptome is the entire repertoire of all transcripts present in a cell at any particular time. We undertook next-generation whole transcriptome sequencing approach to gain insight of the transcriptional landscape of the developing mouse lens. Methods: We ascertained mice lenses at six developmental time points including two embryonic (E15 and E18) and four postnatal stages (P0, P3, P6, and P9). The ocular tissue at each time point was maintained as two distinct pools serving as biological replicates for each developmental stage. The mRNA and small RNA libraries were paired-end sequenced on Illumina HiSeq 2000 and subsequently analyzed using bioinformatics tools. Results: Mapping of mRNA and small RNA libraries generated 187.56 and 154.22 million paired-end reads, respectively

ORGANISM(S): Mus musculus

SUBMITTER: S. Amer Riazuddin 

PROVIDER: E-GEOD-69221 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress