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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Genome-wide analysis of ATF4 binding in histidinol-treated cells

ABSTRACT: Activating Transcription Factor 4 (ATF4) is a transcription factor induced by the integrated stress response (ISR). This experiment is a genome-wide occupancy profiling of ATF4 in human HAP1 cells. HAP1 is a near-haploid human cell line that was derived from KBM-7 cells isolated from a patient with Chronic Myelogenous Leukemia. We induced ATF4 expression by mimicking amino acid starvation with the drug histidinol. We identified peaks of ATF4 binding using three independent antibodies. Examination of ATF4 binding in HAP1 cells treated with 2 mM histidinol for 24 hours.

ORGANISM(S): Homo sapiens

SUBMITTER: Stefan Muljo

PROVIDER: E-GEOD-69304 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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RNA-Seq experiment to identify genes regulated by ATF4

Project description:Activating Transcription Factor 4 (ATF4) is a transcription factor induced by the integrated stress response (ISR). This experiment is a genome-wide profiling of ATF4-dependent RNA expression in human HAP-1 cells. HAP-1 is a near-haploid human cell line that was derived from KBM-7 cells isolated from a patient with Chronic Myelogenous Leukemia. We analyzed WT and ATF4 KO cells. We induced ATF4 expression by mimicking amino acid starvation with the drug histidinol. RNA expression profiles were generated for WT and ATF4 KO HAP1 cells. ATF4 genes were mutated using Cas9 genome editing technology. Amino acid starvation was mimicked by treating WT and ATF4 KO cells with 2 mM histidinol for 24 hours, which increases ATF4 expression.

2015-08-28 | E-GEOD-69308 | biostudies-arrayexpress

Genome-wide analysis of ATF4 binding in histidinol-treated cells

2015-08-28 | GSE69304 | GEO

Genome-wide analysis of ATF4 binding in histidinol-treated cells and RNA-Seq experiment to identify genes regulated by ATF4

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2015-08-28 | E-GEOD-69309 | biostudies-arrayexpress

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Project description:For additional details see Ebert et al, Identification and Small Molecule Inhibition of an ATF4-dependent Pathway to Age-related Skeletal Muscle Weakness and Atrophy. Quadriceps femoris muscles were harvested from 22-month-old muscle-specfic ATF4 knockout (ATF4 mKO) mice and littermate controls. mRNA levels in ATF4 mKO muscles were normalized to levels in littermate control muscles.

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CG methylated microarrays identify novel methylated sequence bound by the CEBPB|ATF4 heterodimer that are active in vivo

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2015-11-19 | E-GEOD-75165 | biostudies-arrayexpress

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