Direct conversion of normal and patient human fibroblasts into neuronal cells

ABSTRACT: Neuronal conversion from human fibroblasts can be induced by lineage-specific transcription factors, holding great promise for human neurological disease modeling and regenerative medicine. However, the introduction of ectopic genes limits their therapeutic applications. Here, we report that neuronal cells could be directly induced from human fibroblasts by a chemical cocktail of seven small molecules bypassing neural progenitor stage. These chemical-induced neuronal cells (hciN cells) resembled hES-derived neurons regarding morphology, gene expression profiles, and electrophysiological properties. Our further experiments show that hciN cells were able to develop into mature neurons in vivo in embryonic mouse brain. Moreover, this approach was further applied to induce hciN cells from fibr

ORGANISM(S): Homo sapiens

SUBMITTER: Wenxiang Hu 

PROVIDER: E-GEOD-69480 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress