Browse
Submit Data
Databases
API
Help

Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

7 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Development of miRNA expression signatures for induction of histone deacetylase inhibitor (part 1)

ABSTRACT: To clarify the relationship between histone acetylation and miRNA expression, we performed miRNA arrays to detect histone deacetylase inhibitor (OBP-801) induced miRNA regulating AR expression in LNCaP and VCaP. Agilent-041686 Unrestricted Human miRNA Microarray (Agilent Technologies, Santa Clara, California) was used according to the manufacturerâ??s protocols.

ORGANISM(S): Homo sapiens

SUBMITTER: Keisuke Katsushima

PROVIDER: E-GEOD-69487 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Development of miRNA expression signatures for induction of histone deacetylase inhibitor (part 2)

Project description:To clarify the relationship between histone acetylation and miRNA expression, we performed miRNA arrays to detect histone deacetylase inhibitor (OBP-801) induced miRNA regulating AR expression in 22Rv1. Agilent-031181 Unrestricted Human miRNA Microarray (Agilent Technologies, Santa Clara, California) was used according to the manufacturerâs protocols.

2016-06-01 | E-GEOD-69488 | biostudies-arrayexpress

Inhibition of Histone Deacetylase induces miR-320-mediated Androgen Receptor Suppression in Prostate Cancer

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2016-06-01 | E-GEOD-69489 | biostudies-arrayexpress

Lysine-Specific Demethylase 1 Has Dual Functions as a Major Regulator of Androgen Receptor Transcriptional Activity

Project description:Lysine Specific Demethylase 1 (LSD1, KDM1A) functions as a transcriptional corepressor through demethylation of histone 3 lysine 4 (H3K4), but has coactivator function on some genes through unclear mechanisms. We show that LSD1, interacting with CoREST, associates with and coactivates androgen receptor (AR) on a large fraction of androgen-stimulated genes. A subset of these AR/LSD1-associated enhancer sites have histone 3 threonine 6 phosphorylation (H3T6ph), and these sites are further enriched for androgen-stimulated genes. Significantly, despite its coactivator activity, LSD1 still mediates H3K4me2 demethylation at these androgen-stimulated enhancers. FOXA1 is also associated with LSD1 at AR regulated enhancer sites, and a FOXA1 interaction with LSD1 enhances binding of both proteins at these sites. These findings show LSD1 functions broadly as a regulator of AR function, that it maintains a transcriptional repression function at AR-regulated enhancers through H3K4 demethylation, and has a distinct AR-linked coactivator function mediated by demethylation of other substrates. Determine the role of LSD1 in androgen signaling.

2014-12-12 | E-GEOD-52201 | biostudies-arrayexpress

Genome-wide analysis of matched microRNA-mRNA time-course data after androgen injection to prostate cancer LNCaP cell line

Project description:Androgen receptor (AR) signalling pathway plays an important role in carcinogenesis and development of prostate cancer. The involvement of microRNA (miRNA) in this process is still largely unknown. In this study, we performed a matched miRNA-mRNA time-course expression profiling to reveal androgen response in hormone-sensitive prostate cancer cells.We introduced novel statistics Response Score (RS) and Modulation Score (MS) to identify significant androgen-regulated target genes and miRNA-modulated target mRNAs. Based on the analysis, we found several novel androgen-regulated targets, which had significant androgen response in expression pattern, and were highly enriched in predicted androgen responsive elements (AREs). AR-bindings to these AREs were validated with ChIP assay. Furthermore, a set of target mRNAs involved in crucial processes of tumor progression were identified to be significantly regulated by these miRNAs. Therefore, a miRNA-mediated androgen signalling network was inferred, including three novel feedback mechanisms for AR self-modulation. In conclusion, our study provides new approaches to further miRNA regulation research and contributes novel findings into miRNA-mediated pathological effects in prostate cancer. Total RNA obtained from androgen dihydrotestosterone (DHT) subjected to LNCaP cells in vitro at 20min, 40min, 1h, 2h, 4h, 8h, 16h, 24h and 48h, compared to the control at 0h.

2011-12-31 | E-GEOD-21245 | biostudies-arrayexpress

Transcription profiling of human prostate cancer cells over-expressing androgen receptor following dihydrotestosterone treament

Project description:dose and time dependent responses of dihydrotestosterone in androgen receptor over-expressing prostate cancer cells.

2009-10-09 | E-MEXP-2286 | biostudies-arrayexpress

Expression analysis of gastric cancer cells treated with periplocin

Project description:To further analyze the change of differential gene expression between SGC-7901 cells treated with 100ng/ml periplocin and untreated cells, respectively. We employed whole genome microarray expression profiling as a discovery platform to identify genes. Gene expression in SGC-7901 cells treated with 100ng/ml periplocin for 24h at 37 ÂºC in a humidified incubator with 5% CO2.

2016-02-23 | E-GEOD-78211 | biostudies-arrayexpress

MicroRNA profiling by array of mouse prostate cancer cell lines treated with dihydrotestosterone and prostate xenografts in intact or castrated mice

Project description:Effect of dihydrotestosterone and castration on microRNA expression

2011-02-25 | E-MEXP-2602 | biostudies-arrayexpress

Genome-wide mapping of retinoblastoma (Rb) binding sites in prostate cancer cell lines VCaP and LNCaP

Project description:Although well characterized as a transcriptional activator, androgen receptor (AR) can also function as a direct transcriptional repressor in prostate cancer cells. The major targets of the AR repressive function are genes mediating DNA synthesis. In this study, we found that AR was recruited to the majority of these DNA synthesis genes and rapidly repressed their transcription. This direct AR mediated repression was enhanced in prostate cancer cells expressing higher levels of AR, and was mediated by recruitment of hypophosphorylated retinoblastoma protein (Rb). Examination of Rb binding in 4 hours DHT treated prostate cancer cell lines VCaP and LNCaP

2015-12-19 | E-GEOD-76141 | biostudies-arrayexpress

miRNA expression data of rat prostate cancer tissue treated with OBP-801, novel HDAC inhibitor

Project description:miRNA expression was obtained from non-treated or OBP-801 treated TRAP rats which develop prostate cancer. The primary aim was to identify miRNAs which regulate prostate carcinogenesis induced by OBP-801.

2016-12-01 | GSE75020 | GEO

Targeting Oct1 genomic function inhibits androgen receptor signaling and castration-resistant prostate cancer growth

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2016-04-01 | E-GEOD-77771 | biostudies-arrayexpress