Delineation of in vitro chondrogenesis of human synovial stem cells following preconditioning with decellularized matrix

ABSTRACT: As a tissue-specific stem cell for chondrogenesis, synovium-derived stem cells (SDSCs) are a promising cell source for cartilage repair. However, a small biopsy can only provide a limited number of cells. Cell senescence from both in vitro expansion and donor age presents a big challenge for stem cell based cartilage regeneration. Here we found that expansion on decellularized extracellular matrix (dECM) full of three-dimensional nanostructured fibers provided SDSCs with unique surface profiles, low elasticity but large volume as well as fibroblast-like shape. dECM expanded SDSCs yielded large pellets with intensive staining of type II collagen and sulfated GAGs, which was supported by both biochemical data and real-time PCR results. Our results also hint at lower levels of inflammatory ge

ORGANISM(S): Homo sapiens

SUBMITTER: Mary Davis 

PROVIDER: E-GEOD-69531 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress