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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of mouse FOG-1-binding defective mutant of GATA-1, ER-GATA-1(V205G), was expressed in GATA-1-null G1E cells

ABSTRACT: Identification of genes regulated by GATA-1 independent of the cofactor FOG-1. Experiment Overall Design: A conditionally activated FOG-1-binding defective mutant of GATA-1, ER-GATA-1(V205G), was expressed in GATA-1-null G1E cells. Transcipt levels were compared in cells untreated or treated with estradiol to activate the GATA-1 mutant.

ORGANISM(S): Mus musculus

SUBMITTER: Kirby Dean Johnson

PROVIDER: E-GEOD-6958 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Friend of GATA-1-independent transcriptional repression: a novel mode of GATA-1 function.

Johnson Kirby D KD Boyer Meghan E ME Kang Jeong-Ah JA Wickrema Amittha A Cantor Alan B AB Bresnick Emery H EH

Blood 20070305 12

The GATA-1-interacting protein Friend Of GATA-1 (FOG-1) is essential for the proper transcriptional activation and repression of numerous GATA-1 target genes. Although FOG-1-independent activation by GATA-1 has been described, all known examples of GATA-1-mediated repression are FOG-1 dependent. In the GATA-1-null G1E cell line, estrogen receptor ligand binding domain (ER) chimeras of either wild-type GATA-1 or a FOG-1-binding defective mutant of GATA-1 repressed several genes similarly upon act ...[more]

PMID: 17339418

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