Landscape of Hematopoiesis Described in Induced Pluripotent Stem Cells and Human Bone Marrow

ABSTRACT: Granulopoietic differentiation of myeloid progenitor cells derived from control iPSCs was performed in a two-step liquid culture. At the end of culture, stages of differentiation were identified by morphological analysis and submitted for RNA-sequencing analysis in order to provide insight into the genomic landscape of myeloid lineage hematopoiesis as modeled by the in vitro induced differentiation of iPSCs as compared to in vivo bone marrow-derived promyelocytes. Peripheral blood from healthy controls was obtained and iPSC were generated from peripheral blood mononuclear cells. Hematopoietic progenitors generated from control iPSCs when cultured in myeloid expansion medium containing 50ng/mL SCF, 10ng/mL IL-3 and 10ng/mL GM-CSF for 5 days at which point cells were stained for CD45-Pacif

ORGANISM(S): Homo sapiens

SUBMITTER: Rebekah Karns 

PROVIDER: E-GEOD-69622 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress