RNA-seq of Brassica rapa leaves elicited with either Pseudomonas syringae pv. maculicola or flg22

ABSTRACT: Phytoalexins are abundant in edible crucifers and have important biological activities, yet no dedicated gene for their biosynthesis is known. Here, we report two new cytochromes P450 from the non-model plant Brassica rapa (Chinese cabbage) that catalyze unprecedented S-heterocyclizations in cyclobrassinin and spirobrassinin biosynthesis. Our results reveal the first genetic and biochemical insights into the biosynthesis of a prominent pair of dietary metabolites, and have important implications for pathway discovery across >20 recently sequenced non-model crucifers. Leaf mRNA profiles of four conditions (Pseudomonas syringae pv. maculicola, flg22, and their respective mock treatments) were tested in triplicate.

ORGANISM(S): Brassica rapa subsp. pekinensis

SUBMITTER: Elizabeth Sattely 

PROVIDER: E-GEOD-69785 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress