An Automated, High Platform for Induced Pluripotent Stem Cell Derivation, Characterization and Differentiation

ABSTRACT: Induced pluripotent stem cells (iPSCs) have become an essential tool for both modeling how causal genetic variants impact cellular function in disease, as well as being an emerging source of tissue for transplantation medicine. Unfortunately the preparation of somatic cells, their reprogramming and the subsequent verification of iPSC pluripotency are laborious, manual processes that limit the scale and level of reproducibility of this technology. Here we describe a modular, robotic platform for iPSC reprogramming that enables automated, high-throughput conversion of skin biopsies into iPSCs and differentiated cells with minimal manual intervention. Using this platform, we demonstrate that automated reprogramming and the pooled selection of pluripotent cells results in high quality, stable,

ORGANISM(S): Homo sapiens

SUBMITTER: Thierry Vilboux 

PROVIDER: E-GEOD-69868 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress