Quantitative transcriptomic analysis of wild type and metabolic mutants of Clostrdidium acetobutylicum ATCC824 under different physiological conditions

ABSTRACT: Transcriptomic analysis was applied to acidogenic, solventogenic and alcohologenic steady-state C. acetobutylicum cells for understanding the regulation of the metabolism of this organism. Used microarray is Clostridium acetobutylicum ATCC824 transcriptional 44k v4, correspondance to GPL10908 Phosphate-limited continuous cultures of C. acetobutylicum were performed under acidogenesis (pH 6.3, 995 mM of carbon from glucose), solventogenesis (pH 4.4, 995 mM of carbon from glucose) and alcohologenesis (pH 6.3, 498 mM of carbon from glucose and 498 mM of carbon from glycerol). Samples were collected for three biological replicates from one control strain and three metabolic mutants, respectively. Total RNA of 36 samples was extracted using an RNeasy Midi kit (Qiagen, Courtaboeuf, France) foll

ORGANISM(S): Clostridium acetobutylicum ATCC 824

SUBMITTER: Philippe Soucaille 

PROVIDER: E-GEOD-69973 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress