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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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ChIP-Seq experiment to analyse Zip1 localisation in the Ctf19c mutants during meiotic prophase.

ABSTRACT: The goal of this experiment is to confirm whether Ctf19c mutants affect Zip1 localisation during meiotic prophase. To do so, diploid S. cerevisiae SK1 cells were constructed containing ndt80-d to arrest cells in prophase. Cells were allowed to sporulate in sporulation media for 5 hours after which formaldehyde was added to the cultures and cells were fixed for 2hr. Samples were then processed according to the standard lab ChIP-Seq protocol (with 3 times 30cycles of 30sec of sonication) Diploid S. cerevisiae SK1 cells were constructed containing ndt80-d to arrest cells in prophase. Cells were allowed to sporulate in sporulation media for 5 hours after which formaldehyde was added to the cultures and cells were fixed for 2hr. Samples were then processed according to the standard lab ChIP-Seq protocol (with 3 times 30cycles of 30sec of sonication)

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Alastair Kerr

PROVIDER: E-GEOD-70029 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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