Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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CaArray_green-00030: 2-methoxyestradiol (2ME2) induces mammary gland differentiation through amphiregulin-EGFR mediated signaling

ABSTRACT: 2-methoxyestradiol (2ME2) induces mammary gland differentiation through amphiregulin-EGFR mediated signaling: molecular distinctions from the mammary gland of pregnant mice.High levels of 2ME2 are observed in the late stages of pregnancy. We investigated the role of 2ME2 on normal mammary gland development. Large scale gene expression assays were performed using Affymetrix GeneChips in pursuit of detailed molecular basis. (1) Mammary glands of wild type FVB mice administered 75 or 150 mg/kg of 2ME2 (2) Mammary glands of normal FVB/Nj mice (i) at day 16 of pregnancy, (ii) day 2 of lactation (iii) day 30 of post-lactation, and (3) mammary epithelial SCp2 cells after 6, 24 and 48 hours of 10 micromol 2ME2 treatment were examined. In vivo studies revealed that 2ME2 treatment up regulates the expression of amphiregulin. The clue to the role of 2ME2 in differentiation comes from studies in vitro which detected down regulation of inhibitor of differentiation (Id-1) gene and consequent up regulation of amphiregulin. The differentiation of E2 negative SCp2 cells by 2ME2 indicate estradiol independent mechanism. For details, please see our paper in Endocrinology 2006. **NOTE: Migrated from caArray 1.x, identifier='gov.nih.nci.ncicb.caarray:Experiment:1015897590892008:1' green-00030 Assay Type: Gene Expression Provider: Affymetrix Array Designs: mg_u74av2, Mouse430_2 Organism: Mus musculus (ncbitax) Tissue Sites: murine mammary gland Material Types: cell, synthetic_RNA, organism_part, total_RNA

ORGANISM(S): Mus musculus

SUBMITTER: Mervi Heiskanen

PROVIDER: E-GEOD-70048 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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2-methoxyestradiol induces mammary gland differentiation through amphiregulin-epithelial growth factor receptor-mediated signaling: molecular distinctions from the mammary gland of pregnant mice.

Huh Jung-Im JI Qiu Ting Hu TH Chandramouli Gadisetti V R GV Charles Rhonda R Wiench Malgorzata M Hager Gordon L GL Catena Raul R Calvo Alfonso A LaVallee Theresa M TM Desprez Pierre-Yves PY Green Jeffrey E JE

Endocrinology 20061207 3

Levels of 2-methoxyestradiol (2ME(2)), an endogenous metabolite of estradiol, are highly elevated during late stages of pregnancy when mammary glands have differentiated with the formation of alveolar structures producing milk proteins. Based upon our previous demonstration that 2ME(2) induces mammary ductal dilation associated with expression of mammary differentiation markers when administered to transgenic mice that spontaneously develop mammary cancer, we studied the effects of 2ME(2) on nor ...[more]

PMID: 17158205

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Dataset Information

CaArray_green-00030: 2-methoxyestradiol (2ME2) induces mammary gland differentiation through amphiregulin-EGFR mediated signaling

Publications

2-methoxyestradiol induces mammary gland differentiation through amphiregulin-epithelial growth factor receptor-mediated signaling: molecular distinctions from the mammary gland of pregnant mice.

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