Ecological transcriptomics: a non-lethal sampling approach for endangered fire salamanders

ABSTRACT: The analysis of transcriptomes is well-established and increasingly affordable in studies at the interface of ecology and evolution. Expression analysis of thousands of genes in parallel reveals functions and pathways involved in relevant phenotypic differentiation. The application of such methods typically involves the sacrifice of the analysed organisms, which is potentially subject to ethical and legal constraints. As an alternative to lethal sampling, transcriptome analyses can be performed using small biopsies of dispensable tissues. It has to be verified, however, to what extent such results are representative of the whole organism. Here, we use a custom microarray to compare transcriptomes of tail-clip samples with those of the remaining whole-body of fire salamander larvae (Salaman

ORGANISM(S): Salamandra salamandra

SUBMITTER: Till Czypionka 

PROVIDER: E-GEOD-70054 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress