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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Nuclear HNRNPA2B1 HITS-CLIP and RNA-seq

ABSTRACT: To identify HNRNPA2B1 binding sites on endogenous nuclear RNAs, we performed HITS-CLIP for endogenous HNRNPA2B1 and RNA-seq to analyze the nuclear RNA under either METTL3 or HNRNPA2B1 depletion. Wild type MDA-MB-231 cells were subjected to the HITS-CLIP procedure on immunoprecipitated HNRNPA2B1 associated RNA obtained from the nuclear fraction (Licatalosi D, et al. 2008, Nature 456:464-U22). For RNA-seq, nuclear RNA was extracted from MDA-MB-231 or Hela cells knocked down for METTL3 or HNRNPA2B1.

ORGANISM(S): Homo sapiens

SUBMITTER: Claudio Alarcon

PROVIDER: E-GEOD-70061 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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