SiRNA profiling of human HeLa cells response to UPF1 depletion

ABSTRACT: Nonsense-mediated mRNA decay (NMD) is a molecular pathway of mRNA surveillance that ensures rapid degradation of mRNAs containing premature translation termination codons (PTCs) in eukaryotes. Originally, NMD was thought of as a quality control pathway that targets non-functional mRNAs arising from mutations and splicing errors. More recently, NMD has been shown to also regulate normal gene expression and NMD thus emerged as one of the key post-transcriptional mechanisms of gene regulation. We have now systematically analyzed the molecular mechanism of variable NMD efficiency and used different HeLa cell strains as a model system. The results of this analysis show that NMD efficiency can be remarkably variable and represents a stable characteristic of these strains. Low NMD efficiency is s

ORGANISM(S): Homo sapiens

SUBMITTER: Marcelo Viegas 

PROVIDER: E-GEOD-7009 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress