Browse
Submit Data
Databases
API
Help

Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

11 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcription profiling of human lymphoblastoid cells following 3 Gy and 10 Gy of ionizing radiation exposure to identify transcriptional response of lymphoblastoid cells to ionizing radiation

ABSTRACT: Time series for gene expression changes following 3 Gy and 10 Gy of ionizing radiation exposure.

ORGANISM(S): Homo sapiens

SUBMITTER: Kuang-Yu Jen

PROVIDER: E-GEOD-701 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Publications

Transcriptional response of lymphoblastoid cells to ionizing radiation.

Jen Kuang-Yu KY Cheung Vivian G VG

Genome research 20030812 9

The effects of ionizing radiation (IR) on the temporal transcriptional response of lymphoblastoid cells were investigated in this study. We used oligonucleotide microarrays to assess mRNA levels of genes in lymphoblastoid cells at various time points within 24 h following gamma-irradiation. We identified 319 and 816 IR-responsive genes following 3 Gy and 10 Gy of IR exposure, respectively, with 126 genes in common between the two doses. A high percentage of IR-responsive genes are involved in th ...[more]

PMID: 12915489

Similar Datasets

Transcriptional response of lymphoblastoid cells to ionizing radiation

Project description:Time series for gene expression changes following 3 Gy and 10 Gy of ionizing radiation exposure. Keywords: time-course

2003-10-08 | GSE701 | GEO

Low dose ionizing radiation treated lymphoblastoid cells

Project description:Irradiated cell lines exposed to 1-10 Gy

2010-07-01 | GSE22639 | GEO

The response to ionizing radiation at the exon level

Project description:Humans are exposed to ionizing radiation (IR) from background radiation, medical treatments, occupational and accidental exposures. IR causes profound changes in transcription. Transcription is a primary process where protein amount and function can be regulated. One aspect of the transcriptional IR response that little is known about on a whole genome basis is alternative transcription. These investigations focus on the response to IR at the exon level in human cells but also at the whole gene level. Whole genome exon arrays were utilized to comprehensively characterize radiation-induced transcriptional expression products in two human cell types, namely EBV-transformed lymphoblast and primary fibroblast cell lines. 12 human primary fibroblast cell lines and 12 primary lymphoblast cell line samples were used for two doses (0 and 10 Gy) and two time points (0 and 4hr). Additional doses ( 1 Gy, 2 Gy, 5 Gy, and 20 Gy) and time points ( 1hr, 2hr, 8hr, 24hr and 48hr) were assessed in four lymphoblast cell lines and four primary fibroblasts.

2011-05-02 | E-GEOD-26841 | biostudies-arrayexpress

Low dose ionizing radiation treated lymphoblastoid cells

Project description:Irradiated cell lines exposed to 1-10 Gy 2 Lymphoblastoid cell lines (GM15510 and GM15036) irradiated 1, 2.5, 5, 7.5, 10 Gy, RNA is isolated and labeled using a T7 amplification Arcturus kit for hybridization on triplicate arrays.

2010-07-30 | E-GEOD-22639 | biostudies-arrayexpress

Exposure to ionizing radiation induced persistent gene expression changes in mouse mammary gland

Project description:Six to eight week old female C57BL/6J mice were exposed to 2 Gy of whole body γ radiation and mammary glands were surgically removed 2-month after radiation. RNA was isolated and microarray hybridization performed for gene expression analysis. 5 samples were analyzed: 2 controls at 2 months, 1 2 Gy at 2 months, and 2 7 Gy at 2 months

2014-05-01 | E-GEOD-57196 | biostudies-arrayexpress

Transcription profiling of human irradiated immortalized B cells, lymphoblastoid cells (CEPH) exposed to 10 Gy of ionizing radiation

Project description:We used microarrays to measure the expression levels of genes in irradiated immortalized B cells, lymphoblastoid cells, from members of Centre dEtude du Polymorphisme Humain (CEPH) Utah pedigrees. Data were collected for cells at baseline and 2 hour and 6 hour after exposure to 10 Gy of ionizing radiation (IR). Experiment Overall Design: We used microarrays to measure the expression levels of genes in irradiated immortalized B cells, lymphoblastoid cells, from members of 15 Centre dEtude du Polymorphisme Humain (CEPH) Utah pedigrees (CEPH 1333, 1341, 1346, 1362, 1408, 1416, 1420, 1421, 1423, 1424, 1444, 1447, 1451, 1454, 1582). Expression data was obtained for cell lines derived from 2 parents and 8 children per each family. Cells were irradiated at 10 Gy in a 137Cs irradiator. Cells were harvested prior to radiation and at 2 and 6 hours following exposure to IR.

2009-04-10 | E-GEOD-12626 | biostudies-arrayexpress

DNA repair genes: Alternative transcription and gene expression at the exon level in response to the DNA damaging agent, ionizing radiation

Project description:DNA repair is an essential cellular process required to maintain genomic stability. Every cell is subjected to thousands of DNA lesions daily under normal changes in transcription. Transcription is a primary process where protein amount and function can be regulated. One aspect of the transcriptional IR response that little is known about on a whole genome basis is alternative transcription. These investigations focus on the response to IR at the exon level in human cells but also at the whole gene level. Whole genome exon arrays were utilized to comprehensively characterize radiation-induced transcriptional expression products in two human cell types, namely EBV-transformed lymphoblast and primary fibroblast cell lines. 12 human primary fibroblast cell lines and 12 primary lymphoblast cell line samples were used for two doses (0 and 10 Gy) and two time points (0 and 4hr). Additional doses ( 1 Gy, 2 Gy, 5 Gy, and 20 Gy) and time points ( 1hr, 2hr, 8hr, 24hr and 48hr) were assessed in four primary lymphoblast cell lines and four primary fibroblasts.

2012-10-26 | E-GEOD-41840 | biostudies-arrayexpress

Radiation response in human cardiac endothelial cells investigated by DIA-MS

Project description:The endothelium is the barrier separating blood and tissue. Radiation-induced enhanced inflammation leading to permeability of this barrier may increase the risk of cardiovascular disease. The aim of this study was to investigate the onset of endothelial inflammatory pathways after radiation exposure. Human coronary artery endothelial cells (HCECest2) were exposed to radiation doses of 0, 0.25, 0.5, 2.0 and 10 Gy (60Co-γ). The cells were harvested 4 h, 24 h, 48 h and 1 wk post-irradiation. The proteomics analysis was performed in a label-free data-independent acquisition mode. The data were validated using bioinformatics and immunoblotting. The low- and moderate-dose-treated samples showed only small proteome changes. In contrast, an activation of DNA-damage repair, inflammation, and oxidative stress pathways was seen after high-dose treatments (2 and 10 Gy). The level of the DNA damage response protein DDB2 was enhanced early at the 10 Gy dose. The expression of proteins belonging to the inflammatory response or cGAS-STING pathway (STING, STAT1, ICAM1, ISG15) increased in a dose-dependent manner showing the strongest effects at 10 Gy after one week. This study suggests a connection between radiation-induced DNA damage and induction of inflammation and supports inhibition of cGAS-STING pathway in the prevention of radiation-induced cardiovascular disease.

2020-11-02 | PXD020735 | Pride

Treatment of primary human astrocytes with 0 Gy or 10 Gy radiation

Project description:Cultured primary human astrocytes were maintained in HA media (SciCell) treated with 0 Gy or 10 Gy radiation to identify changes to gene sets after irradiation.

2021-01-25 | GSE145941 | GEO

Transcriptional response of lymphoblastoid cells to ionizing radiation.

Project description: Not available

| S-EPMC403696 | biostudies-literature