Transcription profiling of mouse C2C12 myoblast cell line to identify oscillatory genes in somitogenesis

ABSTRACT: During somitogenesis, oscillatory expression of genes in the notch and wnt signaling pathways plays a key role in regulating segmentation. These oscillations in expression levels are elements of a species-specific developmental mechanism. To date, the periodicity and components of the human clock remain unstudied. Here we show that a human mesenchymal stem/stromal cell (MSC) model can be induced to display oscillatory gene expression. We observed that the known cycling gene HES1 oscillated with a 5 hour period, consistent with available data on the rate of somitogenesis in humans. We also observed cycling of Hes1 expression in mouse C2C12 myoblasts with a period of 2 hours, consistent with previous in vitro and embryonic studies. Furthermore, we used microarray and quantitative PCR (Q-PCR)

ORGANISM(S): Mus musculus

SUBMITTER: Kenro Kusumi 

PROVIDER: E-GEOD-7012 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress