Reporter Gene Silencing in Targeted Mouse Mutants is Associated with Promoter CpG Island Methylation

ABSTRACT: We report the generation and analysis of high-throughput DNA methylation profiles at nucleotide resolution in a subset of targeted gene trap mouse mutants. Using high-throughput sequencing of bisulfite treated DNA, we generated DNA methylation percentage for CpG islands, and LacZ (reporter) gene in mice with the apparent silencing of the targeted gene promoter reflected by reduced reporter mRNA level. These results were contrasted with findings for a set of mutants with no silencing or CpG methylation following targeted mutagenesis using the same gene trap vector. Our findings supports the hypothesis that presence of the exogenous DNA in the targeting vector may influence the expression of genes in close proximity or may lead to promoter silencing of the target where the promoter is marked

ORGANISM(S): Mus musculus

SUBMITTER: David West 

PROVIDER: E-GEOD-70317 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress