Transcriptome-wide mapping of cut sites of the viral endoribonuclease SOX from Kaposi's sarcoma-associated herpesvirus (KSHV)

ABSTRACT: Purpose: identify sites in endogenous mRNAs that are cut by KSHV SOX; Method: parallel analysis of RNA ends (PARE, following Zhai et al., 2014); Results: SOX cuts at discrete locations in mRNAs human Xrn1 was knocked down in HEK293T cells by shRNAs or siRNAs to stabilize degradation fragments with free 5' ends; GFP-SOX or GFP were transfected for ~24 hrs; total RNA samples were collected and subjected to PARE protocol (Zhai et al., 2014)

ORGANISM(S): Homo sapiens

SUBMITTER: Marta Gaglia 

PROVIDER: E-GEOD-70373 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress