High-throughput sequencing of Moringa oleifera microRNAs: a bioinformatics and functional approach for cross-kingdom regulation

ABSTRACT: MicroRNAs (miRNAs) are endogenous, noncoding, smallRNAs that regulate gene expression at the post-transcriptional level during plant development, growth and seed germination. Among all medicinal plants, Moringa oleifera is one of the most useful trees for which, despite its diffusion, no information about its miRNAs and their respective target genes is available. In this research, we report results obtained from a high-throughput sequencing analysis performed with the Illumina platform. A total of 31,290,964 raw reads were produced from M. oleifera seed smallRNA library. First, we found 99 conserved miRNAs and 43 novel ones that we partially validated by qRT- PCR. Second, by comparing their expression abundances with those of other common plants, we identified 20 conserved M. oleifera miRN

ORGANISM(S): Moringa oleifera

SUBMITTER: Stefano Pirro' 

PROVIDER: E-GEOD-70423 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress