Next generation sequencing facilitates quantitative analysis of control of Candida albicans ATCC 10231 and garlic oil-treated C. albicans ATCC 10231 transcriptomes

ABSTRACT: Purpose: Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goals of this study are to compare transcriptome profiling of control of C. albicans ATCC 10231 (RNA-seq) to transcriptome profiling of garlic oil-treated C. albicans ATCC 10231 and to evaluate protocols for optimal high-throughput data analysis Methods:SDB medium, C. albicans cells and garlic oil were added to two separate 50 mL conical flasks. The initial cell concentrations in culture were both 105 CFU/mL. The cultures were incubated in a water bath shaker at 28 ºC with shaking at 150 rpm for 12 h. Garlic oil or PBS buffer were then added to the cultures to make the concentrations of garlic oil arrived at 0 (control) and 1.25 ?l/mL, respectively. The cultures were continuously i

ORGANISM(S): Candida albicans

SUBMITTER: Wenru Li 

PROVIDER: E-GEOD-70524 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress