TL2_14028s_M9+arabinose_(3_passages)_LibM9_vs_Lib0_Exp.2

ABSTRACT: Salmonella typhimurium 14028s Transposon library recovered after three consecutive rounds of growth to late log phase in M9 minimal medium (arabinose 0.4%) at 37°C with aeration, compared to an expansion of the initial library selected on Luria agar plates + kanamycin (50ug/ml), O/N at 37°C Keywords: Transposon tag analysis 2 biological replicates analysed after arbitrarily primed sampling (Klenow, DOPR degenerate primer) + specific PCR amplification (Taq DNA Pol) + T7 in vitro transcription (AmpliScribe T7) + RT labeling (Cy3) + hybridization, compared to identically processed standard library (Cy5)

ORGANISM(S): Salmonella enterica subsp. enterica serovar Typhimurium

SUBMITTER: Nabil Arrach 

PROVIDER: E-GEOD-7058 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress