Comparing transcriptome by means of DNA microarrays of Streptococcus pneumoniae D39 grown with N-acetylglucosamine to cells grown with the same sugar after a 1 hr pulse with glucose

ABSTRACT: Streptococcus pneumoniae is a strictly fermentative human pathogen that relies on carbohydrate metabolism to generate energy for growth. The nasopharynx colonised by the bacterium is poor in free sugars, but mucosa lining glycans can provide a source of sugar. In blood and inflamed tissues glucose is the prevailing sugar. As a result during progression from colonisation to disease S. pneumoniae has to cope with a pronounced shift in carbohydrate nature and availability. Thus, we set out to assess the pneumococcal response to sugars found in glycans and the influence of glucose (Glc) on this response at the transcriptional, physiological and metabolic levels. Galactose (Gal), N-acetylglucosamine (GlcNAc) and mannose (Man) affected the expression of 8 to 14% of the genes covering cellular fu

ORGANISM(S): Streptococcus pneumoniae D39

SUBMITTER: Tomas Kloosterman 

PROVIDER: E-GEOD-70642 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress