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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Effects of Lrf (encoded by Zbtb7a) deletion on gene expression in mouse embryonic fibroblast (MEF)

ABSTRACT: LRF is reported as a transcription repressor, but its transcription target genes are not completely known We compared the gene expression difference between control and Lrf deleted MEF by microarray Lrfflox/flox MEFs were transduced with MSCV-PIG-Cre or empty control vector retroviruses for 2 days at passage 2. After selection with puromycin for 2 days, total RNAs were purified using the RNAeasy Mini Kit (Qiagen) and treated with RNase-free DNase set (Qiagen). RNAs from two independent experiments were labelled and hybridized using Affymetrix GeneChip® HT Mouse Genome 430 arrays by the Beth Israel Deaconess Medical Center Genomics and Proteomics

ORGANISM(S): Mus musculus

SUBMITTER: Xue-Song Liu

PROVIDER: E-GEOD-70680 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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