Metabolic imbalance caused by loss of malic enzymes in the legume endosymbiont Sinorhizobium meliloti

ABSTRACT: Malic enzymes decarboxylate the tricarboxylic acid (TCA) cycle intermediate malate to the glycolytic end-product pyruvate and are well positioned to regulate metabolic flux in central carbon metabolism. The bacterium Sinorhizobium meliloti has a NAD(P)-malic enzyme (DME) and a NADP-malic enzyme (TME) and DME is required for symbiotic N2-fixation. To help understand the role of these enzymes, we examined growth, metabolic and transcriptional consequences resulting from the deletion of these enzymes. Few effects were observed upon growth with glucose, whereas growth with the gluconeogenic substrate, succinate, resulted in transcriptional and metabolic effects particularly in the dme mutant strains. When grown with succinate, DME mutant cells accumulated hexose sugar phosphates and trehal

ORGANISM(S): Sinorhizobium meliloti

SUBMITTER: Turlough Finan 

PROVIDER: E-GEOD-71308 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress