Unstable Foxp3+ regulatory T cells and altered antigen-presenting cells are associated with lipopolysaccharide-induced fetal loss in pregnant IL10 deficient mice

ABSTRACT: Maternal IL10 deficiency elevates susceptibility to fetal loss induced by the model Toll-like receptor agonist lipopolysaccharide, but the mechanisms are not well elucidated. Here we show that Il10 null mutant (Il10-/-) mice exhibit altered local T cell responses in pregnancy, exhibiting pronounced hyperplasia in para-aortic lymph nodes draining the uterus with >6-fold increased CD4+ and CD8+ T cells compared with wild-type controls. Amongst these CD4+ cells, Foxp3+ Treg cells were substantially enriched, with 11-fold higher numbers at day 9.5 post coitum (pc). Lymph node hypertrophy in Il10-/- mice was associated with more activated phenotypes in dendritic cells and macrophages, with elevated expression of MHCII, scavenger receptor and CD80. Affymetrix microarray revealed an altered trans

ORGANISM(S): Mus musculus

SUBMITTER: John Schjenken 

PROVIDER: E-GEOD-71494 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress