Sexual polyploidization in Medicago sativa L.: impact on the phenotype, gene transcription and genome methylation

ABSTRACT: Polyploidization as the consequence of 2n gamete formation is a prominent mechanism in plant evolution. Studying its effects on the genome and its expression has both basic and applied interest. We crossed two diploid (2n=2x=16) M. sativa plants, a subsp. falcata seed parent and a coerulea x falcata pollen parent that produce a mixture of n and 2n eggs and pollen, respectively. Such cross produced full-sib diploid and tetraploid (2n=4x=32) progenies, the latter being the result of bilateral sexual polyploidization (BSP). These unique materials allowed us to investigate the effects of BSP, and separating the effect of intraspecific hybridization from those of polyploidization by comparing 2x with 4x full sib progeny plants. SSR marker segregation demonstrated tetrasomic inheritance for all

ORGANISM(S): Medicago sativa

SUBMITTER: Alberto Ferrarini 

PROVIDER: E-GEOD-71559 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress