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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Determining the effects of the loss of sympathetic drive in bone, hypothalamus, and gut in adrenergic B1 and B2 receptor knockout mice [bone]

ABSTRACT: Eight weeks old male Adrb1tm1BkkAdrb2tm1Bkk/J , stock number 003810, were purchased from The Jackson Laboratory. These mice are homozygous null for the Adrb1 and Adrb2 genes, and are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Mice were euthanized, and the whole bone marrow was extracted using established methods.1 Whole bone marrow cells from Adrb1tm1BkkAdrb2tm1Bkk/J mice were reconstituted into lethally- irradiated (950 Rad) C57BL/6J mice using a single retro-orbital injection at a ratio of 1:4 (Adrb1tm1BkkAdrb2tm1Bkk/J to C57BL/6J). All reconstituted mice were recovered for 2-3 months prior to tissue collection for mRNA arrays (1) The FASEB Journal 2015:29(1),652.13. Eight control animals and eight animals that received new bone marrow from double adrenergic receptor knockout (treated KO chimera). Following recovery, tissues that were harvested included bone marrow, brain, and distal colon.

ORGANISM(S): Mus musculus

SUBMITTER: Christopher Martyniuk

PROVIDER: E-GEOD-71631 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Determining the effects of the loss of sympathetic drive in bone, hypothalamus, and gut in adrenergic B1 and B2 receptor knockout mice [hypothalamus]

Project description:Eight weeks old male Adrb1tm1BkkAdrb2tm1Bkk/J , stock number 003810, were purchased from The Jackson Laboratory. These mice are homozygous null for the Adrb1 and Adrb2 genes, and are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Mice were euthanized, and the whole bone marrow was extracted using established methods.1 Whole bone marrow cells from Adrb1tm1BkkAdrb2tm1Bkk/J mice were reconstituted into lethally- irradiated (950 Rad) C57BL/6J mice using a single retro-orbital injection at a ratio of 1:4 (Adrb1tm1BkkAdrb2tm1Bkk/J to C57BL/6J). All reconstituted mice were recovered for 2-3 months prior to tissue collection for mRNA arrays (1) The FASEB Journal 2015:29(1),652.13. Eight control animals and eight animals that received new bone marrow from double adrenergic receptor knockout (treated KO chimera). Following recovery, tissues that were harvested included bone marrow, brain, and distal colon.

2016-07-01 | E-GEOD-71633 | biostudies-arrayexpress

Determining the effects of the loss of sympathetic drive in bone, hypothalamus, and gut in adrenergic B1 and B2 receptor knockout mice [distal colon]

Project description:Eight weeks old male Adrb1tm1BkkAdrb2tm1Bkk/J , stock number 003810, were purchased from The Jackson Laboratory. These mice are homozygous null for the Adrb1 and Adrb2 genes, and are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Mice were euthanized, and the whole bone marrow was extracted using established methods.1 Whole bone marrow cells from Adrb1tm1BkkAdrb2tm1Bkk/J mice were reconstituted into lethally- irradiated (950 Rad) C57BL/6J mice using a single retro-orbital injection at a ratio of 1:4 (Adrb1tm1BkkAdrb2tm1Bkk/J to C57BL/6J). All reconstituted mice were recovered for 2-3 months prior to tissue collection for mRNA arrays (1) The FASEB Journal 2015:29(1),652.13. Seven control animals and seven animals that received new bone marrow from double adrenergic receptor knockout (treated KO chimera). Following recovery, tissues that were harvested included bone marrow, brain, and distal colon.

2016-07-01 | E-GEOD-71632 | biostudies-arrayexpress

Determining the effects of the loss of sympathetic drive in bone, hypothalamus, and gut in adrenergic B1 and B2 receptor knockout mice [bone]

2016-07-01 | GSE71631 | GEO

Determining the effects of the loss of sympathetic drive in bone, hypothalamus, and gut in adrenergic B1 and B2 receptor knockout mice [hypothalamus]

2016-07-01 | GSE71633 | GEO

Determining the effects of the loss of sympathetic drive in bone, hypothalamus, and gut in adrenergic B1 and B2 receptor knockout mice [distal colon]

2016-07-01 | GSE71632 | GEO

Expression data from mouse bone marrow adipocytes with age

Project description:The aim of this study was to characterize the age-related gene expression profiles between bone marrow adipocytes and peripheral white adipocytes. Alterations of gene expression with aging were analyzed in bone marrow and peripheral white adipocytes isolated from C57BL/6J male mice using Affymetrix Mouse Gene 1.0 ST arrays. Bone marrow adipocytes and peripheral white adipocytes (n=6-10 animals per group) were isolated from male C57BL/6J mice (6-months, 14-months and 18-months of age). Samples were grouped into cell type (bone marrow adipocytes vs. peripheral adipocytes) and age (6-month (6M), 14-month (14M) and 18-month (18M)).

2011-11-29 | E-GEOD-25905 | biostudies-arrayexpress

Expression data of bone marrow and peripheral adipocytes from leptin deficient mice and obese mice fed with a high fat diet

Project description:The aim of this study was to characterize the obesity-related gene expression profiles between bone marrow adipocytes and peripheral white adipocytes from obese mice fed with high fat diet and leptin deficient mice Alterations of gene expression with high fat diet and in mice lacking leptin were analyzed in bone marrow and peripheral white adipocytes isolated from C57BL/6J male mice using Affymetrix Mouse Gene 1.0 ST arrays. Bone marrow adipocytes and peripheral white adipocytes (n=6-10 animals per group) were isolated from male C57BL/6J mice (6-months, 14-months ) fed with either standard chow or a high fat diet containg 60% calories from fat. Samples were grouped into diet (standard chow vs. high fat diet) and age (6-month (6M), 14-month (14M) and 18-month (18M)).

2011-11-29 | E-GEOD-27017 | biostudies-arrayexpress

Renal gene expression after acute kidney injury in the absence and presence of bone marrow CCR2

Project description:We compared renal gene expression after ischemic reperfusion injury in mice reconstituted with bone marrow with or without myeloid CCR2 prior to injury.

2022-04-14 | GSE193469 | GEO

Expression data from primary bone marrow-derived wild-type C57BL/6 and bcl6 knockout macrophages

Project description:We used microarrays to compare gene expression changes in macrophages caused by deletion of bcl6. Keywords: BCL6 deletion compared to wild-type Primary bone marrow-derived macrophages were isolated from C57BL/6 mice transplanted and reconstituted with wild type or bcl6 knockout bone marrow.

2012-06-07 | E-GEOD-16527 | biostudies-arrayexpress

Bone marrow TTP deficeincy significantly increased inflammation while reduced plasma lipid and hepatic steatosis in LDLR knockout mice

Project description:In order to examine the effects of bone marrow TTP deficiency on inflammation and lipid metabolism and determine the driving force during atherosclerosis, 8 week-old female LDLR−/− mice on C57BL/6J background were lethally irradiated and reconstituted with a wild type (TTP+/+) or TTP knockout (TTP−/−) bone marrow cells and fed a Western diet for 12 weeks. Then mice were sacrificed and tissues were collected for analysis. Liver tissues were collected and gene expression was analysed using a whole genome microarray. Bone marrow TTP−/− recipients displayed a significantly higher systemic and multi-organ inflammation compared to the BM-TTP+/+ recipients. BM-TTP−/− mice displayed a significant reduction of serum lipid levels, attenuated hepatic steatosis, and decreased lipid excretion than the control mice. microarray data showed that BM-TTP deficiency modulate liver genes involved in lipid metabolism and inflammatory response. RT-qPCR was used to confirm the results in several genes from microarray data. BM-TTP deficiency did not further accelerate atherorogenesis process in LDLR−/− mice after a Western diet feeding. TTP promoted VLDL to drive proinflammatory mechanism into proatherogenic mechanism.

2019-08-12 | GSE126481 | GEO

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