Stickleback embryos use ABC transporters as a buffer against exposure to maternally derived cortisol

ABSTRACT: Purpose: Offspring from females that experience stressful conditions during reproduction often exhibit altered phenotypes and many of these effects are thought to arise due to increased exposure to maternal glucocorticoids. Here we used RNAseq to test whether the embryonic transcriptional response to increased levels of cortisol was similar to the transcriptional response to maternal stress in sticklebacks Methods: Eggs were treated by immersing eggs for 30 mins in water that contained 0, 5, or 10 ug/L, which represented baseline, predator induced, and super physiological levels of maternally derived cortisol. To isolated enough RNA for RNAseq, 10 embyros from each clutch/treatment were pooled prior to RNA extraction for a total of 18 pools (6 clutches x 3 treatments). Results: Analysis of

ORGANISM(S): Gasterosteus aculeatus

SUBMITTER: Alison Bell 

PROVIDER: E-GEOD-71649 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress