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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Effect of Smyd1 conditional knockout on gene expression in skeletal muscle

ABSTRACT: Transcriptome analysis by RNA-seq of tibialis anterior muscle from control and Smyd1 myocyte-specific conditional knockout mice at 6 weeks of age. Smyd1 is a methyltransferase specifically expressed in striated muscle and CD8+ T cells. Smyd1 deficiency resulted in centronuclear myopathy primarily affecting fast-twitch muscle fibers. These results provide insight into how loss of Smyd1 altered transcriptional programs resulting in centronuclear myopathy. 6 animals per group (control and Smyd1 conditional knockout)

ORGANISM(S): Mus musculus

SUBMITTER: M. David Stewart

PROVIDER: E-GEOD-71679 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Mouse myofibers lacking the SMYD1 methyltransferase are susceptible to atrophy, internalization of nuclei and myofibrillar disarray.

Stewart M David MD Lopez Suhujey S Nagandla Harika H Soibam Benjamin B Benham Ashley A Nguyen Jasmine J Valenzuela Nicolas N Wu Harry J HJ Burns Alan R AR Rasmussen Tara L TL Tucker Haley O HO Schwartz Robert J RJ

Disease models & mechanisms 20160301 3

The Smyd1 gene encodes a lysine methyltransferase specifically expressed in striated muscle. Because Smyd1-null mouse embryos die from heart malformation prior to formation of skeletal muscle, we developed a Smyd1 conditional-knockout allele to determine the consequence of SMYD1 loss in mammalian skeletal muscle. Ablation of SMYD1 specifically in skeletal myocytes after myofiber differentiation using Myf6(cre) produced a non-degenerative myopathy. Mutant mice exhibited weakness, myofiber hypotro ...[more]

PMID: 26935107

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Effect of Smyd1 conditional knockout on gene expression in skeletal muscle

Project description:Transcriptome analysis by RNA-seq of tibialis anterior muscle from control and Smyd1 myocyte-specific conditional knockout mice at 6 weeks of age. Smyd1 is a methyltransferase specifically expressed in striated muscle and CD8+ T cells. Smyd1 deficiency resulted in centronuclear myopathy primarily affecting fast-twitch muscle fibers. These results provide insight into how loss of Smyd1 altered transcriptional programs resulting in centronuclear myopathy.

2016-04-05 | GSE71679 | GEO

Dysregulated gene expression in Mef2a knockout mice subjected to cardiotoxin-induced muscle injury

Project description:We identified genes expressed in mouse skeletal muscle, during the process of muscle regeneration after injury, which are dysregulated in the absence of Mef2a expression. MEF2A is a member of the evolutionarily conserved MEF2 transcription factor family which has known roles in cardiac muscle development and function, but is not well studied in skeletal muscle. We performed a comparison of gene expression profiles in wild type and MEF2A knockout tibialis anterior muscle, seven days post-injury with cardiotoxin. The results indicated that a variety of genes expressed during muscle regeneration, predominantly microRNAs in the Gtl2-Dio3 locus, are dysregulated by the loss of MEF2A expression. Skeletal muscle RNA used in the present study included the following two sample groups: (WT) pooled total RNA from tibialis anterior muscle taken from 5 wild type mice at seven days post-injury with 10uM cardiotoxin; (KO) pooled total RNA from tibialis anterior muscle taken from 5 Mef2a knockout mice at seven days post-injury with 10uM cardiotoxin. All mice were between 2-4 months of age. Both male and female mice were used.

2013-02-12 | E-GEOD-41871 | biostudies-arrayexpress

Drosophila temperature sensitive col4a1 mutant (DTS-3) and control (OreR) comparison at permissive and at restrictive temperature

Project description:We have recently reported an allelic series of dominant, conditional, temperature-sensitive (DTS) mutations in the type IV collagen gene col4a1 in Drosophila and the onset of severe myopathy by massive degradation of striated muscle fibers, degeneration of epithelial and circular smooth muscle cells of the gut. In order to get a closer look at the molecular causes of the observed phenomena we have executed microarray experiments with the aforementioned mutant. Two growth temperatures for both DTS-3 and OreR flies with dye-swap technical control replicates.

2014-01-31 | E-GEOD-52579 | biostudies-arrayexpress

Drosophila temperature sensitive col4a1 mutant (DTS-3) and control (OreR) comparison at permissive and at restrictive temperature

2014-01-31 | GSE52579 | GEO

Mus musculus

Project description:Effect of Smyd1 conditional knockout on gene expression in skeletal muscle

| PRJNA291783 | ENA

Profiling of glycerol- and CTX-induced models of muscle regeneration in mice

Project description:Utilizing glycerol and cardiotoxin (CTX) injections in the tibialis anterior muscles of M. musculus provides models of skeletal muscle damages followed by skeletal muscle regeneration. In particular, glycerol-induced muscle regeneration is known to be associated with ectopic adipogenesis. We characterized genome-wide expression profiles of tibialis anterior muscles from wild-type mice injured by either glycerol or CTX injection. Our goal was to detect gene expression changes during the time course of glycerol-induced and CTX-induced muscle regeneration models, that can lead to ectopic adipocyte accumulation. Tibialis anterior muscle of 12 week old wildtype C57BL/6J male mice injected i.m with either glycerol or CTX were collected 3, 7, 14 or 21 days after injection. 6 biological replicates were used for each time points. Please note that a few replicate samples did not pass QC test, thus, were removed from the submission. Unchallenged tibialis anterior muscles from a group of 5 wildtype C57BL/6J mice were also as a control. Total RNA was extracted, QCed and hybridized to Affymetrix microarrays.

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2013-03-01 | E-GEOD-27933 | biostudies-arrayexpress

Effect of ATF4 gene knockout on skeletal muscle mRNA levels in fasted mice.

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2012-09-15 | E-GEOD-39193 | biostudies-arrayexpress

Differential gene expression analysis of follistain treated skeletal muscle

Project description:RNA-Seq analysis was performed to define the associated changes in gene expression of skeletal muscle treated with follistatin Skeletal muscle mRNA profiles from follistatin and control treated tibialis anterior muscles. Acute (3 day treatment, 3 control and 4 follistatin replicates) and chronic (7or 14 day treatment, 3 control and 4 follistatin replicates) timepoints were analysed.

2016-09-03 | E-GEOD-78965 | biostudies-arrayexpress

RNAseq of murine myoblast used as cell type control

Project description:Cells isolated from tibialis anterior muscle of mice model, and purified to Integrin7+; VCAM-1; Sca-1- and Lin- progenitors, and place onto culture dishes for 2-3 days before harvest for total RNA isolation

2018-09-16 | E-MTAB-7164 | biostudies-arrayexpress

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