Sphingobium sp. SYK-6 treated with several carbon sources II
Ontology highlight
ABSTRACT:
ORGANISM(S): Sphingobium sp. SYK-6
SUBMITTER: Hirofumi Hara
PROVIDER: E-GEOD-71788 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
Similar Datasets
Project description:Transcriptional profile of RNA extracted from Sphingobium sp. SYK-6 treated with 10 mM dehydrodiconiferyl alcohol (DCA), 10 mM vanillate, or SEMP (10 mM sucrose, 10 mM glutamate, 0.34 mM methionine, and 10 mM proline) comparing labeled SYK-6 genomic DNA. Dual channel experiment, RNA from cells treated with DCA, vanillate, or SEMP vs. genomic DNA. 3 control of genomic DNA, independently grown and harvested.
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Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series
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Project description:Transcriptional profile of RNA extracted from Sphingobium sp. SYK-6 treated with 10 mM dehydrodiconiferyl alcohol (DCA), 10 mM vanillate, or SEMP (10 mM sucrose, 10 mM glutamate, 0.34 mM methionine, and 10 mM proline).
2015-08-07 | GSE71788 | GEO
Project description:Transcriptional profile of RNA extracted from Sphingobium sp. SYK-6 treated with 10 mM dehydrodiconiferyl alcohol (DCA), 10 mM vanillate, or SEMP (10 mM sucrose, 10 mM glutamate, 0.34 mM methionine, and 10 mM proline) comparing labeled SYK-6 genomic DNA.
2015-08-07 | GSE71787 | GEO
Project description:Transcriptional profile of RNA extracted from Sphingobium sp. SYK-6 treated with 10 mM pinoresinol (PR), 10 mM acetovanillone (AV), 10 mM phenylcoumarane (PC), 10 mM vanillate (VA), 10 mM vanillin (VN), 10 mM protocatechuate (PCA), 10 mM fellulate (FA), 10 mM 5,5'- dehydrodivanillate (DDVA), 10 mM 1,2‐bis(4‐hydroxy‐3‐methoxyphehyl)‐1,3‐propanediol (B1), 10 mM syringate (SA), 10 mM guaiacylglycerol‐β‐guaiacyl ether (GGE) comparing RNA extracted from SYK-6 treated with SEMP (10 mM sucrose, 10 mM glutamate, 0.34 mM methionine, and 10 mM proline).
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Project description:Double-strand breaks (DSBs) can lead to the loss of genetic information and cell death. Consequently, cells in all domains of life have evolved mechanisms to repair DSBs, including through homologous recombination. Although recombination has been well characterized, the spatial organization of this process in living cells remains poorly understood. Here, we introduced site-specific DSBs in Caulobacter crescentus, and then used time-lapse microscopy to visualize the homology search, DSB repair, and the resegregation of chromosomal DNA. Even loci tethered to opposite cell poles can efficiently release, pair to enable recombination-based repair, and then resegregate to their original locations. Resegregation occurs independent of DNA replication and without disrupting global chromosome organization. Origin-proximal regions are resegregated by the same machinery, ParABS, used to segregate undamaged chromosomes following DNA replication. In contrast, origin-distal regions efficiently resegregate after a DSB independent of ParABS, and likely without dedicated segregation proteins. Instead, we propose that a physical, spring-like force drives the resegregation of origin-distal loci after DSB repair. Caulobacter cells were depleted of DnaA for 1.5 h before synchronization. Swarmer cells were then released into DnaA depleting conditions (without IPTG) and double-strand breaks were induced for 1 h by the addition of 500 ?M vanillate. For control sample, no vanillate was added. Formadehyde (Sigma) was then added to the final concentration of 1%. Formadehyde crosslinks protein-DNA and DNA-DNA together, thereby capturing the structure of the chromosome at the time of fixation. Fixation was performed at the cell density of OD600 = 0.2. The crosslinking reactions were allowed to proceed for 30 minutes at 25 °C before quenching with 2.5 M glycine at a final concentration of 0.125 M. Fixed cells were then pelleted by centrifugation and subsequently washed twice with 1x M2 buffer (6.1 mM Na2HPO4, 3.9 mM KH2PO4, 9.3 mM NH4Cl, 0.5 mM MgSO4, 10 ?M FeSO4, 0.5 mM CaCl2) before resuspending in 1x TE buffer (10 mM Tris-HCl pH 8.0 and 1 mM EDTA) to a final concentration of 107 cells per µl. Resuspended cells were then divided into 25 µl aliquots and stored at -80 °C for no more than 2 weeks. Each Hi-C experiment was performed using two of the 25 µL aliquots. Chromosome conformation capture with next-generation seqeuncing (Hi-C) was carried out exactly as described previously (Le et al., 2013 PMID: 24158908)
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Project description:An ability to sense and respond to changes in extracellular phosphate is critical to the survival of most bacteria. For Caulobacter crescentus, which typically lives in phosphate-limited environments, this process is especially crucial. Like many bacteria, Caulobacter responds to phosphate limitation through a conserved two-component signaling pathway called PhoR-PhoB, but the direct regulon of PhoB in this organism is unknown. Here, we use ChIP-Seq to map the global binding patterns of the phosphate-responsive transcriptional regulator PhoB in both phosphate-limited and -replete conditions. Combined with genome-wide expression profiling, our work demonstrates that PhoB is induced to regulate nearly 50 genes in phosphate-starved conditions. The PhoB regulon is comprised primarily of genes known or predicted to help Caulobacter scavenge for and import inorganic phosphate, including 15 different membrane transporters. We also investigated the regulatory role of PhoU, a widely conserved protein proposed to coordinate phosphate import with expression of the PhoB regulon by directly modulating the histidine kinase PhoR. However, our studies show that it likely does not play such a role in Caulobacter as depleting PhoU has no significant effect on PhoB-dependent gene expression. Instead, cells lacking PhoU exhibit a striking accumulation of large polyphosphate granules suggesting that PhoU participates in controlling intracellular phosphate metabolism. RNA was collected from cultures grown to mid-exponential phase in rich medium (PYE) at 30 degrees C. The strain grown in the presence of 50 mM vanillate was compared to the same strain grown in the absence of vanillate.
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Project description:p-Hydroxycinnamates, such as p-coumarate and ferulate, are components of plant cell walls and have a number of commercial applications. Previously, we had shown that the soil Actinobacterium Rhodococcus jostii RHA1 (RHA1) grows on ferulate, catabolizing it via vanillate and the β-ketoadipate pathway. We used transcriptomics to identify genes in RHA1 that were specifically up-regulated during growth on ferulate. These include three operons predicted to encode the uptake and β-oxidative deacetylation of ferulate and p-coumarate: couHLT, couMNO and couR. A couL mutant did not grow on p-coumarate, ferulate or their dihydro derivatives, but grew on vanillate. Purified CouL catalyzed the thioesterification of several p-hydroxycinnamates. Among the tested substrates, the best were p-coumarate and caffeate (kcat/KM ~400 mM-1s-1), and sinapate was not transformed. Of these, p-coumarate was also RHA1’s preferred growth substrate. Although the data indicate that p-hydroxycinnamates are catabolized via β-oxidation, the pathway lacks a typical β-ketothiolase. The data further suggest the involvement of two formaldehyde detoxification pathways in vanillate catabolism. This study augments our understanding of the bacterial catabolism of biomass and facilitates the production of aromatics from renewable feedstocks.
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