Mitotic Transcriptional Clearance of Actively Engaged Pol II Through Transcriptional Elongation Control

ABSTRACT: Chromatin is highly condensed and transcriptionally repressed during mitosis. Although it is established that some general transcription factors are inactivated by phosphorylation at mitosis, many details of mitotic transcriptional repression and its underlying mechanisms are largely unknown. Here, we provide evidence that as cells enter mitosis, genes with transcriptionally engaged RNA Polymerase II (Pol II) can continue transcription until the end of the gene to clear Pol II from mitotic chromatin. Using ChIP-Seq, we find that the transcriptional reinitiation process is globally impaired in early mitosis (prophase/prometaphase), with loss of TFIIB occupancy and nucleosome-free regions at promoters. Pretreatment of nocodazole-arrested mitotic cells with the P-TEFb inhibitor flavopiridol p

ORGANISM(S): Homo sapiens

SUBMITTER: Ali Shilatifard 

PROVIDER: E-GEOD-71848 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress