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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Microarray analysis of the skin of P2.5 K5cre-caNrf2 mice

ABSTRACT: The Nrf2 transcription factor is a key player in the cellular stress response, which regulates the expression of important antioxidant enzymes and other cytoprotective proteins. We recently generated a novel transgenic mouse model to determine the function of Nrf2 in the skin. These mice show reduced number of apoptotic keratinocytes after UVB irradiation due to enhanced ROS detoxification. They also show enhanced tumorigenesis in the HPV8 tumor model. RNA from whole skin of 3 single P2.5 K5cre-caNrf2 (tg/tg) and 3 single P2.5 K5cre-wt (tg/wt=control) mice were used

ORGANISM(S): Mus musculus

SUBMITTER: Matthias Schäfer

PROVIDER: E-GEOD-71910 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Functional analysis of the cytoprotective transcription factor Nrf2 in skin morphogenesis and disease: Identification of Nrf2 target genes

Project description:The Nrf2 transcription factor is a key player in the cellular stress response, which regulates the expression of important antioxidant enzymes and other cytoprotective proteins. We recently generated a novel transgenic mouse model to determine the function of Nrf2 in the skin. These mice revealed interesting phenotypic abnormalities, including hyperkeratosis and acanthosis. To gain insight into the underlying molecular mechanisms, we wanted to identify genes, which are differentially expressed in the skin of wild-type and mutant mice before the onset of phenotypic abnormalities. RNA from whole skin of 3 single P2.5 K5cre-caNrf2 (tg/tg) and 3 single P2.5 K5cre-wt (tg/wt=control) mice were used

2012-02-01 | E-GEOD-35160 | biostudies-arrayexpress

Functional analysis of the cytoprotective transcription factor Nrf2 in keratinocytes: Identification of Nrf2-regulated miRNAs

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2015-01-01 | E-GEOD-52454 | biostudies-arrayexpress

Identification of dysregulated genes after deletion of miR-1-1 and miR-1-2

Project description:Purpose: The goals of this study are to identify dysregulated mRNAs in the heart after deletion of miR-1-1 and miR-1-2. Methods: Total RNAs were extracted from hearts at embryonic E15.5 (E15.5) or postnatal 2.5 days (P2.5) of miR-1s dHET and miR-1s dKO mice. mRNAs were purified using a poly-A selection approach and sequenced using Illumina HiSeq 2000. The sequence reads were aligned to the mouse reference genome (NCBI Build 37/mm9) using TopHat program (Bowtie algorithm). Transcript assembles and identification of differentially expressed genes were achieved using Cufflinks package. To account for expression bias due to transcript length, each sample transcript expression was normalized by using cufflinks algorithm with a FDR of 0.05. Results: Using 1.5-fold change as a cutoff, 997 and 653 transcripts were found to be upregulated and downregulated, respectively, in miR-1s dKO heart at P2.5. 423 transcripts were found to be upregulated and 653 were down-regulated in miR-1s dKO heart at E15.5. Many upregulated genes are directly involved in a fetal gene program. Conclusions: miR-1 directly represses a fetal gene program. We performed RNA deep-seq using postnatal 2.5 days (P2.5) heart from miR-1-1 and miR-1-2 double knockout mice and compared it to that of littermate control.

2014-03-26 | E-GEOD-45760 | biostudies-arrayexpress

Mus musculus

Project description:Microarray analysis of the skin of P2.5 K5cre-caNrf2 mice

| PRJNA292435 | ENA

Microarray analysis of the skin of P2.5 K5cre-caNrf2 mice

2015-08-11 | GSE71910 | GEO

Expression data from primary isolated colonic epithelial cells from ChopIEC Tg/Tg mice and Chopflox/flox mice as wild-type controls

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mRNA expression data from BCR-ABL, MYC and CD22DE12 transgenic mouse models

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Transgenic overexpression of Pla2g10 display alopecia and epidermal hyperplasia

Project description:Microarray gene profiling of skin from PLA2G10-Tg mice in comparison with that from control mice yielded data for explaining the overall tendency of Tg skin to show hair follicle distortion and epidermal hyperplasia associated with hyperkeratosis in the absence of inflammation Pla2g10-Tg/+ mice and littermate controls (C57BL/6 background); 25-day old; skin; pooled from 4 mice for each genotype.

2015-08-08 | E-GEOD-71827 | biostudies-arrayexpress

Effect of a 91 day long stay in weightlessness on the International Space Station on mouse skin physiology

Project description:Comparitive gene expression in skin between mice maintained in microgravity (0g) and normogravity (1g) environment. Six male C57Bl/J10 mice were housed for 91 days in the specially designed \Mouse Drawer System\ in weightlessness aboard the International Space Station. Three wild-type mice (WT) and three transgenic mice overexpressing the osteogneic factor PTN/OSF1 under the control of the human bone specific ostecalcin promoter (Tg) were used in the experiment. During the 3-month stay on the ISS, 3 mice unfortunately died leaving 2 Tg and 1 WT. \MDS tissue sharing program\ allowed several teams to study various tissues from these mice. Our aim was to investigate the effect of such a long period of microgravity on skin physiology by morphological, biochemical and genomewide analyses by comparison to similar mice on ground. Gene expression in the skin of 3 space mice and of 3 ground mice was analyzed by microarray. As this unique experiment performed on 3 mice limits the power of statistical analyis, as the transgene PTN/OSF1 was not overexpressed in skin and as a pair wise Pearson's correlation rates between the individual levels of expressed transcripts in the WT and the Tg mice were not significantly different from each other in one experimental group (space or ground), data from the 3 mice were combined to compare results from the space an ground groups.

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Functional analysis of the cytoprotective transcription factor Nrf2 in skin morphogenesis and disease: Identification of Nrf2 target genes

2012-02-01 | GSE35160 | GEO

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