RNA-seq analysis of acute Rb1 deletion in adult mouse tissues

ABSTRACT: Abstract:The pRb tumor suppressor protein associates with chromatin and regulates gene expression. Numerous studies have identified Rb-dependent RNA signatures, but the proteomic effects of Rb-loss are largely unexplored. We have acutely ablated Rb in adult mice and conducted quantitative analysis of RNA and proteomic changes in colon and lung, where Rb-loss was sufficient or insufficient to induce ectopic proliferation respectively. As expected, RbKO caused similar increases in classic pRb/E2F-regulated transcripts in both tissues, but unexpectedly their protein products increased only in the colon, consistent with its increased proliferative index. Thus, these protein changes induced by Rb-loss are coupled with proliferation, but uncoupled from transcription. The proteomic changes in com

ORGANISM(S): Mus musculus

SUBMITTER: Robert Morris 

PROVIDER: E-GEOD-71924 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress