Induced genomic rearrangements and chromothripsis by micronucleus-mediated chromosome transfer [850K]

ABSTRACT: Chromosome segregation errors have been linked to DNA damage and genomic rearrangements. Accumulating evidence has shown that catastrophic genomic rearrangements, like chromothripsis, can result from lagging chromosomes undergoing aberrant DNA replication and DNA damage in micronuclei. Detailed characterization of genomic rearrangements resulting from DNA damage in micronuclei has been hampered because of difficulties in culturing daughter cells with DNA damage. Here, we employ a method by which a specific single chromosome is trapped in a micronucleus, followed by transfer to an acceptor cell. Next, stably propagating clonal cell lines with an extra chromosome were established and analyzed by copy number profiling and whole genome sequencing. While non-transformed, p53 proficient and te

ORGANISM(S): Homo sapiens

SUBMITTER: Wigard Kloosterman 

PROVIDER: E-GEOD-71928 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress