Cross platform standardization of an experimental pipeline for use in the identification of dysregulated human circulating miRNAs

ABSTRACT: In this study we present an experimental pipeline that takes into consideration sample collection, processing, enrichment, and the subsequent comparative analysis of circulating small ribonucleic acids using small RNA sequencing and RT-qPCR. Initially, a panel of miRNAs dysregulated in circulating blood from breast cancer patients compared to healthy women were identified using small RNA sequencing. MiR-320a was identified as the most dysregulated miRNA between the two female cohorts. Total RNA and enriched small RNA populations (<30 bp) isolated from peripheral blood from the same female cohort samples were then tested using a miR-320a RT-qPCR assay. When total RNA was analyzed with this miR-320a RT-qPCR assay, a 2.3-fold decrease in expression levels was observed between blood samples fr

ORGANISM(S): Homo sapiens

SUBMITTER: Tim Downing 

PROVIDER: E-GEOD-72080 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress