Fasting protects mice from lethal DNA damage by promoting small intestinal epithelial stem cell survival

ABSTRACT: Short-term fasting protects mice from lethal doses of chemotherapy through undetermined mechanisms. Herein, we demonstrate that fasting preserves small intestinal (SI) architecture by maintaining SI stem cell viability and SI barrier function following exposure to high-dose etoposide. Nearly all SI stem cells were lost in fed mice, whereas fasting promoted sufficient SI stem cell survival to preserve SI integrity after etoposide treatment. Lineage tracing identified multiple SI stem cell populations, marked by Lgr5, Bmi1 or HopX expression, that contributed to fasting-induced survival. DNA repair and DNA damage response genes were elevated in SI stem/progenitor cells of fasted etoposide-treated mice, which importantly correlated with faster resolution of DNA double strand breaks and less a

ORGANISM(S): Mus musculus

SUBMITTER: Jinsheng Yu 

PROVIDER: E-GEOD-72122 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress