Transcription profiling of human epidermal keratinocytes (NHEK) from neonatal foreskin treated with IL20 subfamily cytokines vs. controls suggests potential roles in cutaneous innate defense and pathogenic adaptive immunity in psoriasis

ABSTRACT: Normal human epidermal keratinocytes (NHEK) from neonatal foreskin were cultured in serum-free EpiLife medium with human KC growth supplement (0.2% bovine pituitary extract (v/v), 5ug bovine insulin, 5ug/ml bovine transferrin, 0.5ng/ml human EGF, and 0.18 ug/ml hydrocortisone) from Cascade Biologics. Cultures were treated with recombinant cytokines from R&D Systems. J Immunol. 2007 Feb 15;178(4):2229-40. Experiment Overall Design: NHEK were treated with IL-19, IL20, IL-22, and IL24, with controls untreated, along with IL1b, IFN gamma, and KGF.

ORGANISM(S): Homo sapiens

SUBMITTER: Kenneth Jung 

PROVIDER: E-GEOD-7216 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress